| rhEPO-Fc融合蛋白质量标准的研究 |
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| 引用本文： | 商阿丽,唐亮,曹传平,武雪宾,郑玲莉,何俊民,鞠佃文.rhEPO-Fc融合蛋白质量标准的研究[J].科技资讯,2011(19):121-122,124. |
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| 作者姓名： | 商阿丽 唐亮 曹传平 武雪宾 郑玲莉 何俊民 鞠佃文 |
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| 作者单位： | 1. 同济大学生命科学与技术学院,上海,200092
2. 上海美恩生物技术有限公司,上海,201203 |
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| 基金项目： | 科技部中小企业创新基金项目 |
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| 摘 要： | 目的：本研究采用的rhEPO-Fc融合蛋白是首次将编码人IgG Fc段的基因与编码人EPO的基因片段连接,并将其克隆至高表达质粒载体中,用该质粒转染CHO细胞进行分泌表达所得。通过对rhEPO-Fc融合蛋白的质量检验和实验方法参数的选择,建立rhEPO-Fc融合蛋白的质量标准。方法：采用细胞MTT比色法及Human EPO ELISA试剂盒测定rhEPO-Fc的体外生物学活性,Lowry法测定蛋白含量,SDS-聚丙烯酰胺凝胶电泳法测定相对分子质量,高效液相色谱法（HPLC）测定纯度,紫外分光光度法测定紫外
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| 关 键 词： | rhEPO-Fc 免疫球蛋白Fc段 融合蛋白 质量标准 |
Quality standards of rhEPO-Fc |
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| SHANG A-li,TANG Liang,CAO CHUAN-ping,et al.Quality standards of rhEPO-Fc[J].Science & Technology Information,2011(19):121-122,124. |
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| Authors: | SHANG A-li TANG Liang CAO CHUAN-ping |
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| Institution: | SHANG A-li,TANG Liang,CAO CHUAN-ping,et al(Shanghai Medipharm Biotech Co.Ltd,Shanghai 201203,China) |
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| Abstract: | Objective:To establish the quality control standards for rhEPO-Fc based on optimized testing methods.Methods:The bioactivity of rhEPO-Fc was analyzed by MTT colorimetric assay and Human EPO ELISA kit,the Lowry method was used to measure the protein concentration,the reduced SDS-PAGE was used to determine the relative molecular weight,the protein purity was analyzed by HPLC and the non-reduced SDS-PAGE,the ultraviolet absorption spectrum was recorded by ultraviolet spectroscopy,the high sensitivity hybridiza... |
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| Keywords: | rhEPO-Fc Fc fragment of immunoglobulin G Fusion protein Quality standards |
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