移植前AS-30D肝癌细胞状态决定其移植后在大白鼠体内的生长周期

在SpragueDawley(SD)大鼠身上测定AS 30D肝癌细胞系转移前的状态。不同来源的细胞 :a)体内 ,来自其他移植的大白鼠 ,b)体外 ,来自培养的细胞 ,和c)冷冻贮存 ,来自液氮储存罐 ,被分别移植入 5周的雌性SD大鼠体内 (数目分别为 5、3、5 )。每只鼠腹腔注射 0 5mL细胞混悬液 ,包含 1 5× 10 6个细胞。结果显示 ,从细胞移植的液体体积 ,细胞浓度 ,总细胞数 ,细胞生存能力和每日食物以及水的消费量等质量参数来看 ,每组白鼠之间没有明显不同。但是 ,如果用其他的细胞代替体内细胞移植到大白鼠的腹腔 ,细胞潜伏期将延长一半或 5d。所以 ,移植前…

Pre-transplant Status of AS-30d Hepatoma Determines in Vivo Growth Period after Transplantation in Sprague-dawley Rat

Objective Pre-transplant status of AS-30D hepatoma cell line was evaluated in Sprague Dawley rats. Cells from different sources, a) in vivo, from the other transplanted Sprague-Dawley rats, b) in vitro, from cultured cell, and c) cryopreservation, from the liquid nitrogen storage tank, were transplanted into 5 week-old female Sprague-Dawley rats (respectively n = 5, 3, and 5). Each animal received intraperitoneal injection of 0.5 ml cell suspension containing 1.5× 106 cells. The results showed no significant difference among groups, in the quality parameters of cell transplantation,such as fluid volume, cell concentration, total cell, cell viability, and the daily consumption of food and water. However, the cell growth duration following the transplantation was lengthened at least one-half fold or 5 days, when other sources of cells than the in vivo, were transplanted into rat abdominal cavity. Thus, the variety of pre-transplant status of the AS-30D cells would enhance flexibility in cell preparations for the successful transplantation in different time frame.