碳源对芽胞杆菌碱性纤维素酶合成的影响

芽胞杆菌ＥＶ２３基本为组成性地合成碱性纤维素酶，以不同碳源生长时酶合成水平差别不显著。葡萄糖（Ｇｌｕ）、果糖等易代谢碳源在ρ＝１０ｇ·Ｌ－１时，不但不抑制酶的合成，还相对地促进酶的合成。以Ｇｌｕ为碳源培养，ρ＝４０ｇ·Ｌ－１时酶合成水平最高，ρ≥５０ｇ·Ｌ－１时酶合成才有部分被阻遏。ＡＴＰ、三羧酸循环中间物对酶合成有一定的阻遏作用，表明ＥＶ２３碱性纤维素酶的抗阻遏合成是有一定限度的。

Effect of Carbon Sources on the Alkaline Cellulase Synthesis

Bacillus sp EV23 synthesized alkaline CMCase almost constitutively in quantity,rather than exerting repressible effect,some ready metabolizable substrates at the mass concentration of 10 g·L-1 stimulated cellulase synthesis relatively.When glucose was used as carbon source, the mass concetration of 40 g·L-1, repressed partially when the level reached at least 50 g·L-1.The presence of ATP and intermidiates in TCA caused the repression of CMCase formation to some extent.