| 文昌鱼eIF5A基因的克隆和进化学分析 |
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| 引用本文： | 王利凤,黄向炜,林浴霜,李忻怡,张红卫.文昌鱼eIF5A基因的克隆和进化学分析[J].山东大学学报(理学版),2004,39(6):112-116. |
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| 作者姓名： | 王利凤 黄向炜 林浴霜 李忻怡 张红卫 |
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| 作者单位： | 山东大学,生命科学学院,山东,济南,250100 |
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| 基金项目： | 国家自然科学基金资助项目(30070094,30270693) |
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| 摘 要： | 对文昌鱼神经胚cDNA文库进行测序和系统分析，首次从青岛文昌鱼(Brachiostoma belcheri tsingtauense)中成功得到1个eIFSA基因的cDNA序列，分析其推导的氨基酸序列揭示该基因产物具有eIF-5A家族蛋白质共有的保守区．对其二级结构进行预测，序列同源性分析表明eIF-5A家族基因在所有的真核生物中都高度保守．进化生物学的分析显示文昌鱼的eIF5A基因与脊椎动物的同源性较高，
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| 关 键 词： | eIF5A 基因 进化 文昌鱼 |
| 文章编号： | 1671-9352(2004)06-0112-04 |
| 修稿时间： | 2004年5月13日 |
Cloning and phylogenetic analysis of eIF5A in ampnioxus |
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| WANG Li-feng,HUANG Xiang-wei,LIN Yu-shuang,LI Xin-yi & ZHANG Hong-wei.Cloning and phylogenetic analysis of eIF5A in ampnioxus[J].Journal of Shandong University,2004,39(6):112-116. |
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| Authors: | WANG Li-feng HUANG Xiang-wei LIN Yu-shuang LI Xin-yi & ZHANG Hong-wei |
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| Institution: | WANG Li-feng,HUANG Xiang-wei,LIN Yu-shuang,LI Xin-yi & ZHANG Hong-wei* |
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| Abstract: | By sequencing and analysing a Qingdao amphioxus (Brachiostoma belcheri tsingtauense) neurulae cDNA library, the cDNA sequence containing a complete open reading frame of the eIF5A gene was obtained. The deduced amino acid sequence was analyzed and compared with the other homologues. We found it with a high conservation domain containing the lysine to be modified to hypusine that shared with in all eIF5A family members. It showed that the eIF5A protein was conserved in almost eukaryotes from the predication of the secondary structure of amphioxus eIF5A protein comparing with other species. The phylogenetic analysis showed a higher conservation of the amphioxus eIF5A with the vertebrate. |
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| Keywords: | eIF5A gene evolution amphioxus |
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