| 番茄高效再生体系的建立 |
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| 引用本文： | 仇 燕,刘红霄,杨松烨.番茄高效再生体系的建立[J].河北科技大学学报,2012,33(1):83-88. |
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| 作者姓名： | 仇 燕 刘红霄 杨松烨 |
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| 作者单位： | 河北科技大学生物科学与工程学院,河北石家庄,050018 |
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| 摘 要： | 以砧木1号番茄的子叶和下胚轴为外植体进行离体组织培养,研究了IAA和6-BA及NAA和6-BA不同激素配比对外植体诱导不定芽及生根的影响。结果表明:培养基MS+0.2mg/L IAA+1.0mg/L 6-BA为诱导子叶外植体愈伤组织和不定芽的最适培养基,而MS+0.5mg/L IAA+1.0mg/L 6-BA为诱导下胚轴外植体愈伤组织和不定芽的最适培养基;诱导不定芽生根的最佳培养基为MS+0.1mg/L IAA。
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| 关 键 词： | 番茄 子叶 下胚轴 植株再生 |
| 收稿时间： | 2011/5/27 0:00:00 |
| 修稿时间： | 2011/11/9 0:00:00 |
Establishment of highly efficient regeneration system of tomato |
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| QIU Yan,LIU Hong-xiao and YANG Song-ye.Establishment of highly efficient regeneration system of tomato[J].Journal of Hebei University of Science and Technology,2012,33(1):83-88. |
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| Authors: | QIU Yan LIU Hong-xiao and YANG Song-ye |
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| Institution: | (College of Bioscience and Engineering,Hebei University of Science and Technology,Shijiazhuang Hebei 050018,China) |
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| Abstract: | The effects of medias containing different concentration of IAA and 6-BA or NAA and 6-BA on tomato callus induction,bud differentiation and rooting were detected in hypocotyl and cotyledon of Zhenmu No.1.The results show that MS+0.2 mg/L IAA+1.0 mg/L 6-BA is the best medium for callus induction and bud differentiation of cotyledon;MS+0.5 mg/L IAA+1.0 mg/L 6-BA is the optimum medium for callus induction and bud differentiation of hypocotyl;the optimum root induction medium is MS +0.1 mg/L IAA. |
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| Keywords: | tomato cotyledon hypocotyl plant regeneration |
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