酸浆对炎症及花生四烯酸代谢途径的影响

探讨酸浆的抗炎镇痛作用及机制. 抗炎实验采用角叉菜胶致大鼠足肿胀和大鼠棉球肉芽肿模型;镇痛实验采用小鼠扭体法. 各模型均随机分为4组:模型组、阳性药吲哚美辛组、酸浆宿萼提取物高剂量组、低剂量组. 测定大鼠足肿胀度,胸腺、脾脏指数,肉芽肿干重,观察小鼠扭体情况,检测足肿胀模型血清中磷脂酶A₂(phospholipaseA₂, PLA₂)、环氧合酶-2(cyclooxygenase 2, COX-2)、前列腺素E₂(prostaglandin E₂, PGE₂)、5-脂氧合酶(5-lipoxygenase, 5-LOX)、白三烯B₄(leukotriene B₄, LTB₄)水平. 实验表明:酸浆宿萼提取物对急、慢性炎症均有显著抑制作用且对机体免疫器官具有一定的保护作用,对急性炎症血清中PLA₂、COX-2、PGE₂、5-LOX、LTB₄均具有抑制作用. 酸浆宿萼提取物可能通过抑制COX-2、5-LOX活性从而双重抑制花生四烯酸(arachidonic acid, AA)代谢途径发挥抗炎作用. 

The Effects of Physalis Alkekengi on Inflammatory and Arachidonic Acid Metabolic Pathway

The objective of this research is to investigate the anti-inflammatory mechanism of Physalis alkekengi. Anti-inflammatory and analgesic effects of Physalis alkekengi calyx extracts were observed by means of carrageenan induced paw edema model, granuloma model and acetic acid writhing test. Rats and mice were divided into four groups randomly as followed: model control group, indomethacin group, Physalis alkekengi calyx extracts high and low dose groups. The foot swelling degree of rats, thymus index, spleen index, the weight of granuloma and the body torsion of mice were observed. The concentrations of PLA₂, 5-LOX, COX-2, PGE₂ and LTB₄ in serum were measured. The results showed that Physalis alkekengi calyx extracts could inhibit acute inflammation and chronic inflammation significantly. Meanwhile, Physalis alkekengi calyx extracts showed a certain protective effect on immune organs and could reduce the contents of PLA₂, COX-2, PGE₂, 5-LOX and LTB₄ in serum in different degrees. The results obtained here showed that Physalis alkekengi calyx extracts might be a dual inhibitor to inhibit AA metabolic pathways by inhibiting the activity of the enzyme COX-2 and 5-LOX oxidation.