| rcan1-1l基因的克隆和表达 |
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| 引用本文： | 刘海朋,杨薇,涂玲辉,魏群,骆静.rcan1-1l基因的克隆和表达[J].北京师范大学学报(自然科学版),2011,47(2):181-184. |
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| 作者姓名： | 刘海朋 杨薇 涂玲辉 魏群 骆静 |
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| 作者单位： | 北京师范大学生命科学学院北京市基因工程药物及生物技术重点实验室; |
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| 基金项目： | 国家自然科学基金资助项目(G30772558;J0630642) |
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| 摘 要： | 从小鼠脑组织中通过总mRNA的提取、RT-PCR方法,利用pEASY-T1载体克隆出了鼠源rcan1-1l基因,并构建出了重组有rcan1-1l基因的PET21a原核表达质粒,进行了表达条件的初步摸索,这为今后研究rcan1-1l基因以及蛋白的结构与功能打下了前期实验基础.
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| 关 键 词： | 钙调神经磷酸酶 rcan1-1l基因 pEASY-T1载体 克隆 表达 |
CLONING AND EXPRESSION OF THE RCAN1-1L GENE |
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| LIU Haipeng,YANG Wei,TU Linghui,WEI Qun,LUO Jing.CLONING AND EXPRESSION OF THE RCAN1-1L GENE[J].Journal of Beijing Normal University(Natural Science),2011,47(2):181-184. |
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| Authors: | LIU Haipeng YANG Wei TU Linghui WEI Qun LUO Jing |
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| Institution: | LIU Haipeng YANG Wei TU Linghui WEI Qun LUO Jing(College of Life Sciences,Beijing Normal University,Beijing Key Laboratory of Genetic Engineering Drugs and Biotechnology: 100875,Beijing,China) |
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| Abstract: | This experiment was aimed at constructing prokaryotic and eukaryotic expression plasmids.Rcan1-l genes were cloned from mouse brain by RT-PCR.Recombinant clones were selected by blue white plaque assay and PCR screen after target gene was subcloned into pEASY-T1 cloning vector.Sequencing confirmed that rcan1-l clone from mouse brain was correct;therefore prokaryotic PET21a plasmid was constructed using restriction enzymes.The right recombinant plasmid was induced to express in expression culture,with the ex... |
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| Keywords: | calcineurin(CN) rcan1-1l gene pEASY-T1 vector clone expression |
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