响应面法优化鬼针草茎中总黄酮的提取工艺

本文以黄酮的提取率为指标,考察乙醇浓度、用量、提取次数、提取时间对提取结果的影响,通过Box-Behnken设计建立响应面模型来优选鬼针草茎中总黄酮的提取工艺参数。结果表明,鬼针草茎中总黄酮最佳提取工艺为乙醇体积分数70%,液料比12∶1(mL/g),提取次数3次,提取时间1.5 h。在此条件下,总黄酮的提取率可达到0.95%,与理论值0.98%的相对误差为3.06%。

Optimization of alcohol extraction techniques of total flavonoids from Bidens bipinnata L. stems using response surface methodology

In this paper, the flavonoids extraction rate was taken as the index, the effects of ethanol concentration, dosage, extraction times and extraction time on the extraction results were investigated, and through Box Behnken design, the response surface model was established to optimize the parameters of the technology for extracting total flavonoids from Bidens bipinnata L. stems. The results showed that the optimum extraction conditions of total flavonoids from Bidens bipinnata L. stems. were as follows: the ethanol volume fraction was 70%, liquid solid ratio was12∶1(mL/g), extracted 3 times, and the extraction time was 1.5 h. In these conditions, the maximum extraction rate of total flavonoids could reach 0.95%, and the relative error was 3.06% when compared with the theoretical value of 0.98%.