1株盐湖噬菌体CJL-7的分离及其生物学特性研究

以嗜盐菌为宿主,从山西省运城盐湖湖水中分离出1株噬菌体,经浓缩纯化后用电镜观察其形态,并进行限制性酶切、蛋白组成分析及生物学特性研究.研究结果表明:分离获得的CJL-7为双链DNA烈性噬菌体,属微小噬菌体科; 其头部为正多面体结构,直径约为80 nm,在其衣壳中至少含3种主要结构蛋白; 该噬菌体含有脂质包膜,宿主范围较广; 低盐处理对该噬菌体的效价几乎没有影响,其活性在30～50 ℃范围内较稳定,在40 ℃时效价最高,在碱性条件下活性更强.

The Isolation and Biological Characteristics of a Bacteriophage CJL-7 from Salt Lake

There are abundant bacteriophages in salt lakes.Isolation and preliminary identification of their biological characteristics will be helpful for further development and utilization.Using halophilic bacteria as the host bacteria,a bacteriophage is isolated from Yuncheng salt lake and named CJL-7 in this study.Then it is observed under electron microscopy after concentration and purification.Finally,restriction enzyme digestion,protein composition analysis and biological characteristics are studied.The results show that CJL-7 is a double stranded DNA virulent phage and belongs to the microphage family.A polyhedron structure is in its head with a diameter of about 80 nm.There are at least three major structural proteins in its capsid.The bacteriophage contains lipid-encapsulated membranes and has a wide host range.Low salt treatment has little effect on its titer.The bacteriophage activity is stable in the range of 30—50 ℃ and has the highest titer at 40 ℃.The activity is enhanced under alkaline conditions.