| 小白芸豆凝集素的分离纯化及性质研究 |
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| 引用本文： | 李锋,王竞,赵晓军.小白芸豆凝集素的分离纯化及性质研究[J].郑州大学学报(理学版),2010,42(1). |
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| 作者姓名： | 李锋 王竞 赵晓军 |
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| 作者单位： | 1. 四川大学,华西医院,四川,成都,610041;中国烟草总公司,郑州烟草研究院,河南,郑州,450001
2. 四川大学,华西医院,四川,成都,610041 |
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| 摘 要： | 从小白芸豆中分离纯化出一种新的凝集素,对其部分理化性质和生物活性进行研究.小白芸豆经过去皮粉碎、浸提、硫酸铵分段盐析、SP Sepharose阳离子交换、Q Sepharose阴离子交换和Superdex 200凝胶过滤层析后可得到小白芸豆凝集素(Phaseolus vulgaris var.albuslectin,PVAL)纯品.测定了PVAL的表观分子质量、亚基分子质量、糖含量、等电点、氨基酸组成、酸碱稳定性以及抗癌性.PVAL的表观分子质量为136 ku,是由2种分子质量分别为36.2 ku和32.2 ku的亚基组成的四聚体,糖含量为4.93%,等电点分布在pH5.3~6.0之间.PVAL分子无半胱氨酸,也无二硫键,疏水性氨基酸质量分数约为37%.PVAL对酸碱的耐受性比较好,并且对肿瘤细胞MDA-MB-435S有较好的抗性.
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| 关 键 词： | 小白芸豆 凝集素 层析 纯化 凝血活性 |
Purification and Properties of a Novel Lectin from Seeds of Phaseolus vulgaris var. albus |
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| LI Feng
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WANG Jing
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ZHAO Xiao-jun.Purification and Properties of a Novel Lectin from Seeds of Phaseolus vulgaris var. albus[J].Journal of Zhengzhou University:Natural Science Edition,2010,42(1). |
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| Authors: | LI Feng WANG Jing ZHAO Xiao-jun |
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| Institution: | LI Feng1,2,WANG Jing1,ZHAO Xiao-jun1(1.West China Hospital,Sichuan University,Chengdu 610041,China,2.Zhengzhou Tobacco Research Institute,CNTC,Zhengzhou 450001,China) |
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| Abstract: | A novel lectin named PVAL from small white kidney beans(Phaseolus vulgaris var.albus) is purified.The purification procedure consists of extracting soluble proteins in 20 mmol/L NaAc buffer(pH 4.8),precipitated with ammonium sulphate,ion-exchange chromatography on SP Sepharose,Q Sepharose and followed by gel filtration on Superdex 200.PVAL is a heterotetramer with molecular mass of 136 ku and composed of two different subunits of 36.2 and 32.2 ku which are held together by noncovalent interactions.The resul... |
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| Keywords: | Phaseolus vulgaris var albus lectin chromatography purification hemagglutinating activity |
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