Cloning and expression pattern of a dehydrin-likeBDN1 gene from drought-tolerantBoea crassifolia Hemsl. |
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| Authors: | Huiwu Zhao Han Liu Haiyuan Yu Yuanlei Hu Yin Gao Zhenyu Li Zhongping Lin |
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| Affiliation: | (1)National Laboratory of Protein Engineering and Plant Genetic Engineering , Peking University, Beijing 100871 ,China;(2)Botanical Institute, Chinese Academy of Sciences, Beijing 100093 ,China |
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| Abstract: | A 500-bp cDNA fragment was amplified via RT-PCR from drought-induced total RNA of the drought-tolerant B. crassifolia Hemsl. using primers based on the sequence of published dehydrin conserved region. By using 5′RACE, full-length coding region (1 148 bp) of BDN1 gene was produced. It is a new member of the dehydrin gene family. Southern analysis indicated that BDN1 is present in the B. crassifolia genome as a single-copy gene. Northern analysis revealed that its expression is inducible by drought and cold stresses as well as ABA application. |
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| Keywords: | drought-tolerant plant Boea crassifolia Hemsl. dehydrin BDN1 gene |
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