丙烯酸丁酯类毛细管液相色谱整体柱的制备与评价

采用甲基丙烯酸丁酯(BMA)为功能单体,己二醇二甲基丙烯酸酯(EDMA)为交联剂,正丙醇、1,4-丁二醇和水为致孔剂,偶氮二异丁腈(AIBN)为引发剂,采用微波辐射的新方法,在毛细管内原位聚合快速制备了毛细管整体柱.扫描电镜结果表明整体材料在毛细管柱中均匀形成并与毛细管内壁结合紧密.采用自组装的毛细管液相色谱仪,优化条件下实现了模型化合物的基线分离.与传统的热聚合和光聚合相比,微波辐射用于有机聚合基质毛细管整体柱的制备是一种更快捷、简单、有效的方法.

Preparation and Evaluation of Methacrylate-based Monolithic Column for Capillary Liquid Chromatography

A methacrylate-based monolith was fabricated inside the fused-silica capillary,which in situ polymerization is rapidly carried out by microwave irradiation.The reactant solution is composed of butyl-methacrylate(BMA)as a functional monomer,ethylene glycol dimethacrylate(EDMA)as a cross-linking agent,1-propanol,1,4-butanediol and water as porogenic solvents and azobisisobutyronitrile(AIBN)as an initiator.The chromatographic performance of the prepared stationary phases is evaluated using the developed capillary liquid chromatography system with a baseline separation of the model analytes.Furthermore,the porosity is determined using the simple flow method and the morphology is characterized by the scanning electron micrograph,which shows that the prepared monolith is stable,forming in the center of the capillary and adhering to the column inner wall.