| 聚合酶链反应检测结核杆菌DNA的质量控制 |
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| 引用本文： | 高小玲,郭天康.聚合酶链反应检测结核杆菌DNA的质量控制[J].甘肃科学学报,2000,12(3):64-66. |
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| 作者姓名： | 高小玲 郭天康 |
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| 作者单位： | 1. 甘肃省临床检验中心,兰州,730000
2. 甘肃省人民医院外科,甘肃,兰州,730000 |
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| 摘 要： | 根据结核杆菌（ＴＢ）ＤＮＡ的提取，耐热性多聚酶（Ｔａｑ）活性、结核Ｌ－型细菌的形成、变异、污染等，阐述了ＴＢ－ＤＮＡ多聚酶链式反应（ＰｏｌｙｍｅｒａｓｅＣｈａｉｎＲｅａｃ－ｔｉｏｎ，ＰＣＲ）检测中假阴性、假阳性出现的原因及内在规律，并提出了相应的改进措施。
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| 关 键 词： | 结核分枝杆菌 PCR 聚合酶链反应 质量控制 DNA |
| 修稿时间： | 1999-11-18 |
FALSE-POSITIVE AND FALSE-NEGATIVE PROBLEMS IN MYCOBACTERIUM TUBERCULOSIS-DNA-PCR DETECTION SYSTEM |
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| GAO Xiao-lin,GUO Tian-kang.FALSE-POSITIVE AND FALSE-NEGATIVE PROBLEMS IN MYCOBACTERIUM TUBERCULOSIS-DNA-PCR DETECTION SYSTEM[J].Journal of Gansu Sciences,2000,12(3):64-66. |
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| Authors: | GAO Xiao-lin GUO Tian-kang |
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| Institution: | GAO Xiao\|lin\+1,GUO Tian\|kang\+2 |
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| Abstract: | Various causes and mechanisms were summed up both of false\|positive and false\|negative results through these aspects of DNA\|extraction, Taq\|DNA polymerase activity, formation of TB L\|type bacterium, variation and pollution,et al, in Mycobacterium tuberculosis\|DNA\|PCR detection system. In view of these, some measures for improvement were suggested. |
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| Keywords: | M tuberculosis Polymerase Chain Reaction(PCR) false\|positive false\|negative |
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