| 丝状真菌产黄青霉DNA提取方法的改进 |
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| 引用本文： | 赵颖,刘静,戴梦,郑桂珍,章丽,张佳,王富强.丝状真菌产黄青霉DNA提取方法的改进[J].河北大学学报(自然科学版),2010,30(4). |
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| 作者姓名： | 赵颖 刘静 戴梦 郑桂珍 章丽 张佳 王富强 |
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| 作者单位： | 华北制药集团新药研究开发有限责任公司,微生物药物国家工程研究中心,河北省工业微生物代谢工程技术研究中心,河北,石家庄,050015 |
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| 基金项目： | 国家科技支撑计划项目 |
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| 摘 要： | 为探索提取产黄青霉基因组DNA的简便方法,采用起始菌体量0.2 g,10 g/L十六烷基三甲基溴化铵裂解液,通过液氮/65℃反复冻融对菌种破壁,代替了液氮研磨,且不需要玻璃珠,操作简便,重复性好,提取的DNA质量高,极大地方便了后续基因操作.
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| 关 键 词： | 基因组提取 液氮冻融 十六烷基三甲基溴化铵 |
An Improved Method of Extracting Genomic DNA from Filamentous Fungus Penicillium chrysogenum |
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| ZHAO Ying,LIU Jing,DAI Meng,ZHENG Gui-zhen,ZHANG Li,ZHANG Jia,WANG Fu-qiang.An Improved Method of Extracting Genomic DNA from Filamentous Fungus Penicillium chrysogenum[J].Journal of Hebei University (Natural Science Edition),2010,30(4). |
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| Authors: | ZHAO Ying LIU Jing DAI Meng ZHENG Gui-zhen ZHANG Li ZHANG Jia WANG Fu-qiang |
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| Abstract: | A method for extraction of genomic DNA from filamentous fungus Penicillium chrysogenum were developed.Mycelia were lysed by 10 g/L CTAB(Hexadecyltrimethylammomum bromide) and a liquid nitrogen/65 ℃ freeze-thaw procedure.The quality of DNA was demonstrated by PCR(polymerase chain reaction).This rapid extraction procedure is a good repeatability method and is suitable for treating many samples at one time,and it provides a method of high throughput strategy. |
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| Keywords: | genomic DNA extraction freeze-thaw CTAB |
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