| A型口蹄疫病毒VP1免疫活性肽基因串联片段的化学合成及克隆与表达 |
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| 引用本文： | 易建中,黄海斌,杨志军,黄妤,邓余,严维耀,丁恩雨,龙永进,徐泉兴,郑兆鑫.A型口蹄疫病毒VP1免疫活性肽基因串联片段的化学合成及克隆与表达[J].复旦学报(自然科学版),1997(5). |
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| 作者姓名： | 易建中 黄海斌 杨志军 黄妤 邓余 严维耀 丁恩雨 龙永进 徐泉兴 郑兆鑫 |
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| 作者单位： | 复旦大学遗传学研究所!上海200433(易建中)，复旦大学(黄海斌，杨志军，黄妤，邓余，严维耀，丁恩雨，郑兆鑫)，上海畜牧兽医所(龙永进，徐泉兴) |
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| 摘 要： | A型口蹄疫病毒（FMDV）VP1蛋白的141～160和200～213位氨基酸序列是决定FMDV免疫原性的抗原决定簇片断．人工合成一个免疫活性肽基因的串连片段（20AA－14AA-20AA）、全部选用大肠杆菌偏爱密码子．在5’端带有EcoR1位点和一个起始密码子ATG，在3’端带有BamH1位点和一个终止密码子TGA．利用这两个酶切位点把该基因连接到pWR590质粒上．并筛选高表达的菌株．结果表明该融合蛋白在大肠杆菌JM101中能高表达．经斑点ELISA实验证明．大肠杆菌细胞中表达的融合蛋白有A型FMDV抗原活性．
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| 关 键 词： | 基因工程疫苗 融合蛋白 串连基因 A型FMDV |
A biosynthetic peptide vaccine against type A strain of foot-and-mouth disease virus |
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| Yi Jianzhong, Huang Haibing, Yang Zhijun, Huang Yu, Deng Yu,Yan Weiyao, Ding Enyu, You Yongjin, Xu Qianxin, Zheng Zhaoxin.A biosynthetic peptide vaccine against type A strain of foot-and-mouth disease virus[J].Journal of Fudan University(Natural Science),1997(5). |
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| Authors: | Yi Jianzhong Huang Haibing Yang Zhijun Huang Yu Deng Yu Yan Weiyao Ding Enyu You Yongjin Xu Qianxin Zheng Zhaoxin |
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| Institution: | Fudan University. Institute of Vetermary Science of Shanghai |
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| Abstract: | A peptide containing residues of amino acids 141~160 and 200~213 of FMDV VPI plays the dominant role in the immune response.A tandem gene fragment (20AA-14AA 20AA)coding for the peptide was chemically sythesized by DNA synthesizer. It is 213bp with an EcoR 1 site at the end and a BamH 1 site at the 3' end. The synthetic fragment was inserted into the plasmid pWR590, an expression vector with lac promotor. A fusinon protein with 3-galactosidasc was highly expressed. Its antigenicity was positive when tested by ELISA. |
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| Keywords: | genetie engineering vaccine fusion protein tandem gene type A strain of FMDV |
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