不同酸处理对罗非鱼皮明胶提取率及物化特性的影响

以罗非鱼皮为原料,探讨不同酸处理对鱼皮明胶蛋白质提取率、羟脯氨酸含量、蛋白质组成和黏度的影响.实验结果表明,鱼皮明胶蛋白质的提取率受溶液中氢离子浓度的影响很大,乙酸、柠檬酸和盐酸处理的鱼皮蛋白质提取率分别为13.50%～20.55%,10.52%～20.01%和4.32%～20.86%.优化3种酸的处理条件,得到最高的蛋白质提取率和羟脯氨酸含量较为接近,但对应的黏度不同.凝胶电泳结果表明,明胶中主要含α-链和β-链,α-链含量高的明胶有更好的黏度特性.

Effects of Different Acid Treatment on Yield and Physicochemical Properties of Gelatin Derived from Tilapia Skin

The influence of different acid treatment on protein yield(PY),hydroxyproline(HYP) content,protein distribution and viscosity of fish gelatin was investigated in this paper.The results showed that proton concentration greatly affected the gelatin extraction rate.The protein yield of acetic acid,citric acid and HCl were 13.50%～20.55%,10.52%～20.01% and 4.32%～20.86%,respectively.The highest protein yield and hydroxyproline content using the three acids were similar,while the corresponding viscosity was different.Gel electrophoresis results showed that the extracted gelatin contained mostly α-chains and β-chains,and the gelatin with more α-chains showed better viscosity properties.