酪氨酸分子印迹电化学传感器的制备及性能

采用恒电位沉积法,制备以L-酪氨酸为模板分子,壳聚糖为功能基体的分子印迹电化学传感器.实验结果表明:在优化的制备和测试条件下,所制得的印迹传感器对L-酪氨酸具有良好的特异识别性能,印迹因子达3.34;在pH值为6.0,0.1 mol·L^-1的磷酸盐缓冲溶液中,L-酪氨酸的微分脉冲伏安氧化峰峰电流与浓度在4.0×10^-7~1.0×10^-4 mol·L^-1范围内呈良好的线性关系,检出限为2.0×10^-7 mol·L^-1.将该传感器用于人血清中酪氨酸含量的测定,平均回收率为89.50%~99.67%.

Preparation and Properties of Tyrosine Molecularly Imprinted Electrochemical Sensor

A novel molecularly imprinted electrochemical sensor was prepared with chitosan as functional matrix and L-tyrosine(L-Tyr)as template molecule by potentiostatic electrodeposition. According to our experimental results, the molecular imprinted polymer(MIP)sensor with an imprinted factor of 3.34 showed well sensitivity and good selectivity to L-Tyr among the structural similarities and co-existences. The oxidation peak currents of differential pulse voltammetry were linear to the concentration of L-Tyr in the range from 4.0×10^-7 to 1.0×10^-4 mol·L^-1 with a detection limit of 2.0×10^-7 mol·L^-1. Furthermore, the recoveries ranged from 89.50% to 99.67% were obtained when the sensor was applied to real samples analysis.