| 响应曲面法优化酸奶中嗜热链球菌H1的生长条件 |
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| 引用本文： | 王玉芹,周晶,沙未来. 响应曲面法优化酸奶中嗜热链球菌H1的生长条件[J]. 山东理工大学学报：自然科学版, 2013, 0(6): 26-31 |
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| 作者姓名： | 王玉芹 周晶 沙未来 |
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| 作者单位： | 曲阜师范大学生命科学学院,山东曲阜273165 |
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| 基金项目： | 国家级大学生创新创业训练计划项目（201210446007）;山东省自然科学基金资助项目（ZR2010CL014）;山东省高等学校科技计划项目（J12LE02）;曲阜师范大学青年基金资助项目（XJ201107,XJ201001）. |
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| 摘 要： | 采用单因素试验和响应曲面法对酸奶中嗜热链球菌H1的最适生长条件进行了研究和优化.根据回归分析得到的最适培养条件为:温度41.02℃,pH5.74,接种量0.31mL·50mL-1,盐浓度0.23%,此时菌株的光密度OD600为1.176.经实验验证,在该条件下菌株光密度OD600为1.162,其相对误差小于2%.
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| 关 键 词： | 嗜热链球菌 响应曲面法 生长条件 优化 |
Optimization of growth condition for S. thermophilus H1 by response surface methodology |
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| WANG Yu-qin,ZHOU Jing,SHA Wei-lai. Optimization of growth condition for S. thermophilus H1 by response surface methodology[J]. Journal of Shandong University of Technology:Science and Technology, 2013, 0(6): 26-31 |
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| Authors: | WANG Yu-qin ZHOU Jing SHA Wei-lai |
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| Affiliation: | (College of Life Science, Qufu Normal University, Qufu 273165, China) |
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| Abstract: | The growth conditions of S .thermophilus H1 were studied by single factor experiment and response surface methodology . The results showed that the optimal parameters for the growth conditions as follows ：the temperature was 41 .02℃ ,the pH was value 5 .74 ,the inoculum size was 0 .31mL · 50mL -1 ,the salt concentration was 0 .23% ,and the OD600 of strain HI was 1 . 176 .Under the optimal conditions the OD600 of strain HI was 1 .162 ,The relative error was bel-lo w 2% . |
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| Keywords: | S. thermophilus growth condition response surface methodology optimization |
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