| 两对引物PCR扩增黑斑蛙Sox基因 |
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| 引用本文： | 陈艳秋,杜祥英,相学军,张磊,张新蕊,朱必才.两对引物PCR扩增黑斑蛙Sox基因[J].徐州师范大学学报(自然科学版),2007,25(3):61-64. |
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| 作者姓名： | 陈艳秋 杜祥英 相学军 张磊 张新蕊 朱必才 |
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| 作者单位： | 徐州师范大学,生命科学学院,江苏,徐州,221116 |
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| 基金项目： | 国家自然科学基金;徐州师范大学学生处基金 |
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| 摘 要： | 以人的SRY基因的HMG-box保守区设计的两对引物,对黑斑蛙Sox基因的扩增.结果如下:1)雌雄黑斑蛙均扩增出一条带,大小约为220 bp,说明Sox基因的长度在黑斑蛙雌雄个体间无性别差异;2)两对引物的扩增结果相同,表明Sox基因有高度保守性.
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| 关 键 词： | 黑斑蛙 Sox基因 聚合酶链式反应(PCR) |
| 文章编号： | 1007-6573（2007）03-0061-04 |
| 修稿时间： | 2007-02-07 |
PCR Amplification of Sox Gene of R.nigromaculata Using Two Pairs of Primers |
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| CHEN Yan-qiu,DU Xiang-ying,XIANG Xue-jun,ZHANG Lei,ZHANG Xin-rui,ZHU Bi-cai.PCR Amplification of Sox Gene of R.nigromaculata Using Two Pairs of Primers[J].Journal of Xuzhou Normal University(Natural Science Edition),2007,25(3):61-64. |
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| Authors: | CHEN Yan-qiu DU Xiang-ying XIANG Xue-jun ZHANG Lei ZHANG Xin-rui ZHU Bi-cai |
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| Institution: | School of Life Science, Xuzhou Normal University, Xuzhou, Jiangsu, 221116, China |
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| Abstract: | The Sox gene of R.nigromaculata was amplified using two pairs of primers which specially amplify the conservation sequence of human HMG-box gene.Results show that: 1) The Sox gene exists in both female and male R.nigromaculata,and both the lengths of the fragments of PCR products are 220 bp.It shows that there is no difference in Sox gene between male and female R.nigromaculata;2) Both pairs of the primers have the same products.We can see the evolution of Sox gene. |
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| Keywords: | R nigromaculata Sox gene polymerase chain reaction |
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