| Genetic analysis and molecular mapping of a presenescing leaf gene psll in rice （Oryza sativa L.） |
| |
| 作者姓名： | WANG dun WU Shujun ZHOU Yong ZHOU Lihui XU Jiefen HU Jing FANG Yunxia GU Minghong LIANG Guohua |
| |
| 作者单位： | Key Laboratory of the Ministry of Education for Plant Functional Genomics/Jiangsu Key Laboratory for Crop Genetics and Physiology, Yangzhou University, Yangzhou 225009, China |
| |
| 基金项目： | Acknowledgements This work was supported by the State Key Program of Basic Research of China （Grant No. 30530118）; the National Natural Science Foundation of China （Grant Nos. 2003CB114303 ＆ 2005CB120807）, and the Key Program of the Bureau of Education, Jiangsu, China （Grant No. 05KAJ2012）. |
| |
| 摘 要： | A rice psl1 （presenescing leaf） mutant was obtained from a japonica variety Zhonghua 11 via radiation of ^60Co-γ in M2 generation. Every leaf of the mutant began to wither after it reached the biggest length, while the leaves of the wild variety could keep green for 25--35 d. In this study, genetic analysis and gene mapping were carried out for the mutant identified. The SSR marker analysis showed that the mutant was controlled by a single recessive gene （psl1） located on chromosome 2. Fine mapping of the psl1 locus was conducted with 34 new STS markers developed around psl1 anchored region based on the sequence diversity between Nipponbare and 93-11. The psl1 was further mapped between two STS markers, STS2-19 and STS2-26, with genetic distances of 0.43 and 0.11 cM, respectively, while cosegregated with STS2-25. A BAC contig was found to span the psl1 locus, the region being delimited to 48 kb. This result was very useful for cloning of the psl1 gene.
|
| 关 键 词： | psIl基因 水稻 叶片早衰 遗传分析 分子图谱 |
| 收稿时间： | 2006-04-16 |
| 修稿时间： | 2006-04-162006-09-11 |
Genetic analysis and molecular mapping of a presenescing leaf gene psl1 in rice (Oryza sativa L.) |
| |
| WANG dun WU Shujun ZHOU Yong ZHOU Lihui XU Jiefen HU Jing FANG Yunxia GU Minghong LIANG Guohua.Genetic analysis and molecular mapping of a presenescing leaf gene psl1 in rice (Oryza sativa L.)[J].Chinese Science Bulletin,2006,51(24):2986-2992. |
| |
| Authors: | Jun Wang Shujun Wu Yong Zhou Lihui Zhou Jiefen Xu Jing Hu Yunxia Fang Minghong Gu Guohua Liang |
| |
| Institution: | (1) Key Laboratory of the Ministry of Education for Plant Functional Genomics/Jiangsu Key Laboratory for Crop Genetics and Physiology, Yangzhou University, Yangzhou, 225009, China |
| |
| Abstract: | A rice psl1 (presenescing leaf) mutant was obtained from a japonica variety Zhonghua 11 via radiation of 60Co-y in M2 generation. Every leaf of the mutant began to wither after it reached the biggest length, while the leaves of the
wild variety could keep green for 25–35 d. In this study, genetic analysis and gene mapping were carried out for the mutant
identified. The SSR marker analysis showed that the mutant was controlled by a single recessive gene (psl1) located on chromosome 2. Fine mapping of the psl1 locus was conducted with 34 new STS markers developed around psl1 anchored region based on the sequence diversity between Nipponbare and 93-11. The psl1 was further mapped between two STS markers, STS2-19 and STS2-26, with genetic distances of 0.43 and 0.11 cM, respectively,
while cosegregated with STS2-25. A BAC contig was found to span the psl1 locus, the region being delimited to 48 kb. This result was very useful for cloning of the psl1 gene. |
| |
| Keywords: | rice simple sequence repeat (SSR) marker sequence-tagged site (STS) marker molecular mapping BAC contig |
| 本文献已被 维普 SpringerLink 等数据库收录！ |
| 点击此处可从《中国科学通报(英文版)》浏览原始摘要信息 |
| 点击此处可从《中国科学通报(英文版)》下载免费的PDF全文 |
