| 一株具抗肿瘤活性的北极细菌的筛选及分子鉴定 |
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| 引用本文： | 曾润颖,李根,林昱.一株具抗肿瘤活性的北极细菌的筛选及分子鉴定[J].厦门大学学报(自然科学版),2002,41(6):800-803. |
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| 作者姓名： | 曾润颖 李根 林昱 |
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| 作者单位： | 1. 厦门大学生命科学学院,福建,厦门,361005;国家海洋局第三海洋研究所海洋生物工程重点实验室,福建,厦门,361005
2. 国家海洋局第三海洋研究所海洋生物工程重点实验室,福建,厦门,361005 |
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| 基金项目： | 中国首次北极科学考察研究课题，国家科技部基础性工作项目 (G 99A 15 )资助 |
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| 摘 要： | 在中国首次北极科学考察期间，从北极海域采集了水体，沉积物等样品，从中分离得到一批嗜冷细菌，采用MTT法对这些北极海洋细菌进行了细胞毒活性物质的筛选，得到一株具有细胞毒活性的菌株。采用16SrDNA序列分析及系统发育分析方法对该株菌株进行了分子鉴定。
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| 关 键 词： | 抗肿瘤活性 北极细菌 MTT分析法 细胞毒活性物质 分子鉴定 菌株筛选 海洋微生物 |
| 文章编号： | 0438-0479(2002)06-0800-04 |
| 修稿时间： | 2002年3月7日 |
The Screening and Molecular Identification of Arctic Marine Psychrophilic Microorganism with Anti-tumor Function |
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| ZENG Run-ying ,LI Gen ,LIN Yu.The Screening and Molecular Identification of Arctic Marine Psychrophilic Microorganism with Anti-tumor Function[J].Journal of Xiamen University(Natural Science),2002,41(6):800-803. |
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| Authors: | ZENG Run-ying LI Gen LIN Yu |
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| Institution: | ZENG Run-ying 1,2,LI Gen 2,LIN Yu 2 |
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| Abstract: | A number of psychrophilic microorganisms were isolated from arctic sea water and sediment samples collected during the China First Arctic Investigation cruise. A strain with cell toxicity was isolated by MTT screening model which was used to identify anti-tumor active material. The genera of the strain was identified based on comparison and phylogenetic analysis of 16S rDNA gene sequence. |
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| Keywords: | arctic psychrophilic marine bacteria MTT assay molecular identification |
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