尼罗罗非鱼在淡、海水中Na~+-K~+-ATPase活性变化

用ATPase活性测试盒测定了尼罗罗非鱼鳃和肾中Na+-K+-ATPase活性在海、淡中的变化.从淡水到海水中,尼罗罗非鱼鳃和肾Na+-K+-ATPase活性分别增强了1.61倍和1.41倍;把尼罗罗非鱼由淡水直接放入海水,鳃中的Na+-K+-ATPase活性升高较剧烈,在前期(0.5 h)最高,在鱼即将死亡的后期(8 h)次之,在中期(3 h)较低;肾中Na+-K+-ATPase活性升高较缓慢,在前期较小,中期较高,后期最高.除了在淡水中,肾的Na+-K+-ATPase活性高于鳃外,在海水中以及海水的前期、中期和后期中,鳃的Na+-K+-ATPase活性都高于肾,与肾相比,鳃对肾盐协迫更为敏感.

Changes of Na~+-K~+-ATPase Activity of Oreochromis Niloticus in Freshwater and Seawater

Na~+-K~+-ATPase activity in gill and kidney of Oreochromis niloticus were tested with ATPase test kit when the fish were in freshwater and seawater.Na~+-K~+-ATPase activity in gill in seawater strengthen 1.61 times compared with in freshwater,it is 1.41 times to kidney.When Oreochromis niloticus were transferred from freshwater into seawater directly,Na~+-K~+-ATPase activity in prephase of seawater(0.5 h),in postphase of seawater(8 h)that is the fish is on the point of death,it has a medium values of Na~+-K~+-ATPase activity,in middlephase,Na~+-K~+-ATPase activity is the minimum.Na~+-K~+-ATPase activity in kidney has a different thing,in post-phase of seawater it has the maximum values,in middlephase of seawater it is medium,in prephase of seawater it has the maximum values,in middlephase of seawater it is medium,in prephase of seawater it has a minimum values.Besides in freshwater,kidney has a higher Na~+-K~+-ATPase activity values than gill,in gill,there are higher Na~+-K~+-ATPase activity values than in kidney in seawater,prephase,middlephase and postphase of seawater. Compare to kidney,gill is more sensitive to salt threatener.