肾透明细胞癌组织和细胞系趋化素样因子1的表达及其临床意义

趋化素样因子1(chemokine-like factor 1, CKLF1)在多种恶性肿瘤中发挥了不同的生物学作用,为探讨CKLF1在肾透明细胞癌(clear cell renal cell carcinoma, ccRCC)组织及细胞系中表达水平并分析其临床意义。利用免疫组化法、蛋白质印迹和实时定量PCR法,检测CKLF1在肾透明细胞癌组织和肾癌细胞系中的表达水平,并分析肾透明细胞癌组织中CKLF1表达水平与临床病理Fuhrman分级之间的相关性。免疫组化结果显示:CKLF1在肾透明细胞癌组织和癌旁组织中的表达,差异有显著性(P0.05); CKLF1表达水平的高低在临床病理各分级之间无明显差异(P0.05)。Western blot结果显示:CKLF1蛋白在3种细胞系ACHN、Caki、HKC中的表达差异有统计学意义(P0.05);qRT-PCR结果显示,CKLF1 mRNA在3种细胞系ACHN、Caki、HKC中的表达差异有统计学意义(P0.05)。CKLF1的表达在肾透明细胞癌组织中和肾癌细胞系ACHN、Caki中明显上调,CKLF1是一个潜在的肾透明细胞癌分子标志物。

Expression of Chemokine-like Factor 1 in Renal Clear Cell Carcinoma Tissues and Cell Lines and its Clinical Significance

_{Objective Chemokine-like factor 1 (chemokine-like factor 1, CKLF1) plays different biological roles in many malignant tumors. This study aims to investigate and analyze the expression level of CKLF1 gene in renal clear cell carcinoma tissues and cell lines Its clinical significance. Methods: Immunohistochemistry, Western blot and real-time PCR were used to detect the expression level of CKLF1 in clear cell renal cell carcinoma tissues and renal cancer cell lines,and to analyze the correlation between the expression of CKLF1 in renal cancer tissues and the clinical pathological Fuhrman grade.ResultsImmunohistochemical results: The expression of CKLF1 in clear cell renal cell carcinoma and adjacent tissues was significantly different (P<0.05); there was no significant difference in the level of CKLF1 expression between clinicopathological grades (P >0.05). Western blot results showed that the expression of CKLF1 protein in the three cell lines ACHN, and Caki, HKC was significantly different (P<0.05); The results of qRT-PCR showed that the expression of CKLF1 mRNA in the three cell lines ACHN, Caki, and HKC was significantly different (P<0.05).Conclusion The expression of CKLF1 was significantly up-regulated in renal clear cell carcinoma tissues and renal cancer cell lines ACHN and Caki, .CKLF1 is a potential molecular marker of renal clear cell carcinoma.}