重组绿豆LBBI的制备及其对小鼠的辐射防护作用

目的: 研究重组绿豆LBBI蛋白是否对小鼠辐射损伤具有防护作用。方法: 60只BALB/c小鼠分为3组(n=20)，即生理盐水组、重组LBBI注射组和安多霖灌胃组。60 Co射线源对小鼠进行一次性全身照射，照射剂量为6.5 Gy。60 Co照射半小时后，对各组小鼠分别注射生理盐水、LBBI（50.0 mg/(kgd)）和灌胃给药安多霖（3 g/(kgd)），每隔24 h给药一次，连续给药一周，观察小鼠成活率及外周血象恢复的情况。结果: 与生理盐水组小鼠相比，重组LBBI组和安多霖组小鼠的30 d存活率明显增高（P0.01），平均生存时间延长（P0.01），小鼠外周血红细胞、白细胞、血小板数目明显增加（P0.05）。结论: 重组LBBI蛋白具有防止辐射损伤的作用。

Preparation of the recombinant mung bean LBBI and its role in protection of mice from radiation

Objective: To confirm whether the recombinant mung bean LBBI protein has the protective effect of anti-radiation injury for mice. Methods: 60 BALB/c mice were randomly divided into normal saline control, recombinant LBBI and, anduolin group, all of which were irradiated with 60 Co at 6.5 Gy for whole bod. Half an hour latter, the mice in every group received intravenous injection with normal saline and LBBI(50.0 mg/(kgd) )respectively, and intragastric administration with anduolin(3 g/(kgd) for first time. These treatments were carried out for every 24 h for one week. Then, the survival rate and peripheral hemogram recovery of mice were observed. Results: Compared to normal saline group, the 30 days survival rate of group mice injected with LBBI protein was increased significantly （P0.01）,and its average survival time was prolonged（P0.01）. We also confirmed that the number of red blood cell, white blood cells and platelets in LBBI group mice wereincreased significantly than that of normal saline group（P0.05）. Conclusions: The recombinant LBBI can protect mice from radiation injury.