| 不同细胞裂解方法提取活性污泥总DNA研究 |
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| 引用本文： | 曲艳玲,廖永红,汪苹,牟文亨.不同细胞裂解方法提取活性污泥总DNA研究[J].北京工商大学学报(自然科学版),2007,25(2):13-16. |
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| 作者姓名： | 曲艳玲 廖永红 汪苹 牟文亨 |
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| 作者单位： | 北京工商大学,化学与环境工程学院,北京,100037 |
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| 摘 要： | 采用直接提取法对活性污泥总DNA进行提取,以化学机械法为基础,在细胞裂解步骤中,分别设计了化学法与超声法、高温法、反复冻融法、珠磨法及液氮研磨法相结合等方法,并通过DNA样品的浓度、纯度、提取率以及肠杆菌基因间重复共有序列扩增片段多态性等4个指标对活性污泥总DNA提取的不同细胞裂解方法进行比较,确定了反复冻融法为提取效率高、DNA纯度好且适用于PCR扩增的最佳DNA提取方法.
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| 关 键 词： | 活性污泥 细胞裂解 总DNA提取 ERIC-PCR |
| 文章编号： | 1671-1513（2007）02-0013-04 |
| 收稿时间： | 2006-09-22 |
| 修稿时间： | 2006年9月22日 |
COMPARISON OF DIFFERENT DNA EXTRACTION METHODS FOR ACTUVATED SLUDGE |
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| QU Yan-ling,LIAO Yong-hong,WANG Ping,MU Wen-heng.COMPARISON OF DIFFERENT DNA EXTRACTION METHODS FOR ACTUVATED SLUDGE[J].Journal of Beijing Technology and Business University:Natural Science Edition,2007,25(2):13-16. |
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| Authors: | QU Yan-ling LIAO Yong-hong WANG Ping MU Wen-heng |
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| Institution: | College of Chemical and Environmental Engineering, Bussiness University, Beijing 100037, MU Wen-heng Beijing Technology and China |
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| Abstract: | Directed-extraction method was used for DNA extraction from activated sludge.Based on the chemicallysis and Mechinablysis,different DNA extraction methods for activated sludge were evaluated.They are ultrasonicallysis,high temperature extraction,freeze-thawing,bead mill homogenization and liquid nitrogen method.The effects of them were compared based on the concentration,purity,efficiency of distillation of the recovered DNA,and length polymorphism of ERIC-PCR products. |
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| Keywords: | activated sludge fragmentation of cells DNA extraction ERIC-PCR |
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