| HPLC法测定苦荞中多菌灵的残留量 |
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| 引用本文： | 吴浪,张勇,李存雄,赵君,陈顺琴,周永丹.HPLC法测定苦荞中多菌灵的残留量[J].江西师范大学学报(自然科学版),2015,0(3):246-248. |
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| 作者姓名： | 吴浪 张勇 李存雄 赵君 陈顺琴 周永丹 |
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| 作者单位： | 1.贵州师范大学贵州省山地环境信息系统与生态环境保护重点实验室,贵州 贵阳 550001;
2.贵州师范大学化学与材料科学学院,贵州 贵阳 550001;
3.贵州师范学院化学与生命科学学院,贵州 贵阳 550018 |
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| 基金项目： | 贵州省科技厅科学技术基金(黔科合J字LKS[2011]30号);贵州省教育厅自然科学基金(黔教科2010017);贵州省教育厅招标课题(黔教科(2011)018号)资助项目 |
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| 摘 要： | 建立了一种分析苦荞中多菌灵残留量的高效液相色谱法(HPLC).苦荞粉粹后,用乙酸乙酯超声提取,净化、浓缩、用乙腈定容.采用配有紫外检测器的HPLC测定,以乙腈-水(体积比为65:35)为流动相,流速为1.0 mL·min,检测波长为282 nm.浓度在0.025～5.000 μg·mL范围内多菌灵的质量浓度与峰面积线性关系良好,R为0.999 9; 平均回收率为83.60；～91.59；,RSD为2.11；～6.56；,方法的最低检出限(3S/N)为0.01 mg·kg.该方法操作简单,灵敏度、重现性良好.
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| 关 键 词： | 高效液相色谱 苦荞 多菌灵 残留 |
The Determination of Carbendazim Residue in Buckwheat by HPLC |
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| WU Lang
,
ZHANG Yong
,
LI Cunxiong
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ZHAO Jun
,
CHEN Shunqin
,
ZHOU Yongdan.The Determination of Carbendazim Residue in Buckwheat by HPLC[J].Journal of Jiangxi Normal University (Natural Sciences Edition),2015,0(3):246-248. |
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| Authors: | WU Lang ZHANG Yong LI Cunxiong ZHAO Jun CHEN Shunqin ZHOU Yongdan |
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| Institution: | WU Lang;ZHANG Yong;LI Cunxiong;ZHAO Jun;CHEN Shunqin;ZHOU Yongdan;Key Laboratory for Information System of Mountainous Area and Protection of Protection of Ecological Environment of Guizhou Province,Guizhou Normal University;School of Chemistry and Life Science,Guizhou Normal College;School of Chemistry and Material Sciences,Guizhou Normal University; |
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| Abstract: | The method was established to the detect the carbendazim residue in buckwheat by the high performance liquid chromatography(HPLC).The buckwheat samples were splintered,extracted with ethyl acetate by ultrasonic wave,purified,concentrated and diluted with acetonitrile.The experiments were performed by HPLC equipped with VWD.Experiment conditions are as follows:mobile phase,acetonitrile:water(65:35); flow rate,1 mL·min; wavelength,282 nm.The peak area was linearly proportional to carbendazim concentration in the range between 0.025～5.000 μg·mL.The results showed the R was 0.999 9,RSD was 2.11；～6.56；,the average recovery was 83.60；～91.59；,and the lowest detection limit was 0.01 mg·kg.The method has advantages of simply operating,high sensitivity and good reproducibility. |
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| Keywords: | HPLC buckwheat carbendazim residual |
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