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| 多杀性巴氏杆菌幽灵的研究 | |
| 引用本文： | 刘东胜,朱必凤,杨旭夫. 多杀性巴氏杆菌幽灵的研究[J]. 韶关学院学报, 2008, 29(12) |
| 作者姓名： | 刘东胜 朱必凤 杨旭夫 |
| 作者单位： | 1. 南昌大学生命科学学院,江西,南昌,330031;韶关学院英东生物工程学院,广东韶关,512005 2. 韶关学院英东生物工程学院,广东韶关,512005 |
| 摘 要： | 采用基因工程方法,将PhiX174噬菌体裂解基因E和温敏控制表达系统与质粒pPBA1100基因杂交,构建了重组子pPBA1100-E.将重组子转化到多杀性巴氏杆菌中,通过温度诱导使裂解基因表达.用限制性内切酶检验重组子.扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率.结果表明:重组质粒经限制性内切酶酶切鉴定,琼脂糖电泳呈现三条谱带,相对分子质量与理论值一致.扫描电镜观察显示,重组子在多杀性巴氏杆菌中成功表达,获得了细菌幽灵.菌落形成单位检验结果显示,多杀性巴氏杆菌遗传灭活率达到99%. |
| 关 键 词： | 多杀性巴氏杆菌 细菌幽灵 裂解基因E 质粒构建 |
Preliminary study on bacterial ghost of Pasteurella multocida |
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| LIU Dong-sheng,ZHU Bi-feng,YANG Xu-fu. Preliminary study on bacterial ghost of Pasteurella multocida[J]. Journal of Shaoguan University(Social Science Edition), 2008, 29(12) | |
| Authors: | LIU Dong-sheng ZHU Bi-feng YANG Xu-fu |
| Affiliation: | 1.Life science College; Nanchang University; Nanchang 330031; Jiangxi; China; 2.Yingdong College of Biotechnology; Shaoguan University; Shaoguan 512005; Guangdong; China); |
| Abstract: | Recombinant pPBA1100-E was constructed by which the gene E of bacteriophage Phix174 and temperature sensitivity expressing control system hybridized with plasmid pPBA1100 by genetic engineering method.Recombinant was transform to Pasteurella multocida and lysis gene E expressed by temperature inducing.Recombinant was detected by restriction endonuclease.Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi... |
| Keywords: | Pasteurella multocida bacterial ghost lysis gene E plasmid construction |
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