酶改性技术研究

酶具有催化效率高、催化专一性强等显著特点,然而,酶的活力和稳定性往往不能满足应用的要求.为了改进酶的催化特性,笔者及其所在课题组20多年来长期进行酶分子修饰、酶固定化、酶非水相催化、酶定向进化等酶改性技术的研究,发现通过酶的改性,可以显著改进酶活力和稳定性.文中对笔者及其所在课题组在酶改性技术方面的研究成果和进展作了介绍.

Research on Enzyme Improving Technology

Although enzymes possess high catalytic efficiency and good substrate specificity,their activities and stabilities often can not meet the requirements of application.In order to improve the catalytic properties of enzymes,the author and his group have devoted themselves to the researches of enzymatic molecular modification,enzyme immobilization,non-aqueous enzymatic catalysis and directed enzyme evolution in the past two decades,finding that the catalytic properties of enzymes such as activity and stability can significantly be improved by enzyme improving technology.This paper reviews the achievement and progress in the field of enzyme improving technology made by the author and his group.