大鼠海马发育过程中CRMPs家族mRNA的表达

目的:探讨大鼠海马发育过程中坍塌反应调节蛋白(CRMPs)家族mRNA的表达规律.方法:用RT-PCR方法检测大鼠发育不同阶段CRMPs家族mRNA的表达.结果:RT-PCR实际扩增长度与设计长度相吻合.内参照β-actin电泳条带在发育各时期灰度无明显差异.CRMP-1 mRNA以新生鼠和幼年鼠表达最多,成年大鼠弱表达(P<0.05).CRMP-2从胚胎鼠至幼年鼠呈现逐渐增加的趋势,成年鼠相对较低(P<0.05).CRMP-3以幼鼠和成年大鼠表达较多,其中幼鼠表达最多(P<0.05),新生鼠表达最少(P<0.05).CRMP-4在胎鼠、新生鼠和幼年鼠表达量接近,成年鼠表达相对较低(P<0.05).CRMP-5在各个阶段均呈较高表达,以新生鼠和成年鼠表达较多,其中成年大鼠表达最多(P<0.05),胎鼠表达最弱(P<0.05).结论:从胚胎期至幼年期,CRMPs家族除CRMP-4稳定于高表达水平外,其余成员表达量均逐渐增多,其中CRMP-3和CRMP-5区别于其他成员,在成年期表达量继续增高.

Expression of CRMPs family mRNA in the development of rat hippocampus

Aim:To investigate the expression rule of CRMPs family mRNA in the development of rat hippocampus. Methods: The mRNA of CRMPs was detected using RT-PCR. Results: Limpid electro-phoresis strips located in the contrived position. The expression of β-actin was similar in all rats. The most expression of CRMP-1 was in neogentic and juvenile rats, and the least was in adult rats (P< 0.05). The expression of CRMP-2 be inclined to increase gradually from embryonic to juvenile rats, and the adult rats were less than the others(P<0.05). Compared with CRMP-1 and CRMP-2, juvenile and adult rats expressed more CRMP-3, the most was in juvenile rats(P<0.05), but the least was in embry-onic rats(P<0.05). The expression of CRMP-4 was almost in embryonic, neogentie and juvenile rats, but weaker expression in adult rats(P<0.05). The expression of CRMP-5 was more in all period and mainly in the neogentic and adult rats, the most was in adult rats(P<0.05 ), the least was in embryonic (P<0.05). Conclusion:Exclusive of CRMP-4 stabilized high expression, from embryonic to juvenile, the other members had the tendency to raise gradually. CRMP-3 and CRMP-5 kept up higher expression in adult rats among the family.