| 保护剂浓度对第19期鸡胚原始生殖细胞存活率的影响 |
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| 引用本文： | 肖小珺,秦洁,蔡琳琳,李碧春.保护剂浓度对第19期鸡胚原始生殖细胞存活率的影响[J].实验动物科学,2004,21(3):24-26. |
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| 作者姓名： | 肖小珺 秦洁 蔡琳琳 李碧春 |
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| 作者单位： | 扬州大学畜牧兽医学院,扬州,225009 |
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| 基金项目： | 国家自然科学基金 (30 170 6 78)资助项目 |
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| 摘 要： | 采用Ficoll密度梯度离心 ,提取第 19期 (孵化 72h)性腺中的原始生殖细胞 (PGCs) ,用不同浓度的冷冻保护液进行冷冻保存。复苏后的PGCs用台盼蓝染色检测其存活率 ,并进行体外培养。结果发现 :在同一浓度下 ,不同的冷冻保护液之间存在显著 (P <0 0 5 )或极显著 (P <0 0 1)差异。在同一种冷冻保护液下 ,不同的浓度之间存在显著(P <0 0 5 )或极显著 (P <0 0 1)差异 ,具体表现为冷冻保护液浓度越大 ,复苏后细胞的存活率越低。PGCs复苏后于体外培养 ,可增殖形成细胞克隆 ,并可传代培养
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| 关 键 词： | 鸡 性腺 鸡胚 原始生殖细胞 存活率 冷冻保存 体外培养 |
| 文章编号： | 1006-6179(2004)03-0024-03 |
| 修稿时间： | 2003年10月27 |
Cryopreservation of Chicken Primordial Germ Cells at Stage 19 Under Different Preservative Density |
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| XIAO Xiao-jun,QIN Jie,CAI Lin-lin,LI Bi-chun.Cryopreservation of Chicken Primordial Germ Cells at Stage 19 Under Different Preservative Density[J].Shiyan Dongwu Kexue,2004,21(3):24-26. |
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| Authors: | XIAO Xiao-jun QIN Jie CAI Lin-lin LI Bi-chun |
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| Abstract: | Isolating the primordial germ cells(PGCs)from gonads at stage 19 by Ficoll density gradient centrifugation.Cryopreservating the PGCs at difference freezing media density.The vitality of the frozen-thawed PGCs was determined by the Trypan blue exclusion method,than the PGCs were cultured in vitro.The result showed:at the same density,there is significant difference(P<0.05) or very significant difference(P<0.01) among the freezing media.At the same freezing medie,there is significant difference(P<0.05)or very significant difference(P<0.01) among the different density,the density more higher,the vitality more lower.When the thawed PGCs culture in vitro,it can propagation,form PGCs colony,subculture. |
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| Keywords: | Chicken Primordial germ cells Cryopreservation Culture in vitro |
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