首页 | 本学科首页   官方微博 | 高级检索  
     检索      

银杏茎段的组织培养
引用本文:罗紫娟.银杏茎段的组织培养[J].杭州师范学院学报(社会科学版),1990(3).
作者姓名:罗紫娟
作者单位:杭州师范学院生物系
摘    要:取自银杏(Ginkgo biloba Linn)雌雄株上当年抽出的幼嫩茎梢,剪去叶子,把嫩茎切成约0.5—0.8cm的具节小段,分别接种在十种培养基上。3天后切口处膨大;7—10天后,出现愈伤组织,节上的腋芽开始生长;30天左右培养于N_6+MS微量+kH_2PO_(4100)+2.4-D_3+BA_1+NAA_1PPm的培养基上的部分腋芽基部长出根,形成完整的再生植株。

关 键 词:银杏  茎段培养  再生植株培养

TISSUE CULTRUE OF STEM SEGMENTS OF GINKGO BILOBA LINN
Luo Zijuan.TISSUE CULTRUE OF STEM SEGMENTS OF GINKGO BILOBA LINN[J].Journal of Hangzhou Teachers College(Humanities and Social Sciences),1990(3).
Authors:Luo Zijuan
Institution:Department of Biology
Abstract:The young stem tips of male and female Ginkge biloba Linn, were cut into 0.5-0.8cm stem segments and cultivated in ten different kinds of the culture medium. Callus were induced to form in 7-10 days. The axillary buds on the noded segments with nodes began to grow. Then in the culture medium: Ne+MStrace+KH2PO4100 + 2,4-D 3 +BA1+NAA1 PPm, roots were fromed from the bases of the axillary buds in about 30 days. These buds grew up to form complete plants.
Keywords:Ginkgo biloba Linn  tissue cultrue of stem  tissue cultrue regenerating plantlets  
本文献已被 CNKI 等数据库收录!
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号