| 磷酸缓冲液对大豆种子萌发期抗冷性的影响 |
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| 引用本文： | 郑蔚虹,赫延龄,张东向,万翠香,穆庆梅.磷酸缓冲液对大豆种子萌发期抗冷性的影响[J].高师理科学刊,1995(3). |
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| 作者姓名： | 郑蔚虹 赫延龄 张东向 万翠香 穆庆梅 |
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| 作者单位： | 齐齐哈尔师范学院生物系，齐齐哈尔师范学院生物系，齐齐哈尔师范学院生物系，齐齐哈尔师范学院生物系，大庆职工医学院 94届毕业生. |
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| 摘 要： | 用0.01mol/L的pH5.8磷酸缓冲液浸种9h,可在大豆种子萌发期低温胁迫((3±1）℃,24h)条件下,能有效地降低相对电导率,水溶性蛋白含量和稀碱提取蛋白含量明显增加,脯氨酸含量也显著增加.表明磷酸缓冲液浸种对大豆种子萌发期在低温胁迫时,具有防护作用.
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| 关 键 词： | 磷酸缓冲液 大豆 萌发 抗冷性 蛋白质 |
Effect of Phosphate Buffer on the Chilling Injury Resistance of Glycine Max Seed in the Bud |
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| Zheng Weihong He Yanling Zhang Dongxiang Wan,Cuixiang.Effect of Phosphate Buffer on the Chilling Injury Resistance of Glycine Max Seed in the Bud[J].Journal of Science of Teachers＇College and University,1995(3). |
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| Authors: | Zheng Weihong He Yanling Zhang Dongxiang Wan Cuixiang |
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| Institution: | Zheng Weihong He Yanling Zhang Dongxiang Wan Cuixiang(Department of Biology)Mu Qingmei(Daqing Worker-staff Medical Institute) |
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| Abstract: | When 0.01mol/L of phosphate buffer at PH 5.8,was used to soak the glycine max seed for 9 h, it was found that its soaking with phosphate buffer caused remarkable reduction of electrolyte lackage from glycine max seed in the bud during chilling(3±1℃ 24 h ) treatment. In the meantime, the water and thin alkali soluble protein content increased significantly ,and the protein also enhanced. Our results show that the stability of cell merbrance caused by phosphate buffer may explain the chilling injury resistance of glycine max seed in the bud. |
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| Keywords: | Phosphate buffer Glycine max Relative conductivety Bud Chilling injury resistance Protein |
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