| Sequence analysis of mRNA polyadenylation signals of rice genes |
| |
| 作者姓名： | LU Ying GAO Chenxi HAN Bin |
| |
| 作者单位： | [1]National Center for Gene Research, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200233, China [2]Graduate School of the Chinese Academy of Sciences, Beijing 100039, China [3]Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, China |
| |
| 基金项目： | Acknowledgements The authors thank Zhang Yujun, Hu Wenhuo, Zheng Xingnan and Ying Kai for their technical assistance and helpful comments. This work was supported by the Ministry of Science and Technology of China （Grant No. 2002AA2Z1003）, the Chinese Academy of Sciences, and the Shanghai Municipal Commission of Sciences and Technology （Grant No. 038019315）. |
| |
| 摘 要： | Most eukaryotic mRNAs receive a poly (A) tail at their 3′-ends through a process involving the cleavage of pre-mRNA and the concomitant polymerization of adenosine residues to the cleaved RNA end1,2]. As un- translated regions (UTRs) may contain importa…
|
| 关 键 词： | 多聚腺苷酸 序列分析 mRNA 水稻 基因工程 |
| 文章编号： | 10.1007/s11434-006-1069-5 |
| 收稿时间： | 2006-01-17 |
| 修稿时间： | 2006-01-172006-02-16 |
Sequence analysis of mRNA polyadenylation signals of rice genes |
| |
| LU Ying GAO Chenxi HAN Bin.Sequence analysis of mRNA polyadenylation signals of rice genes[J].Chinese Science Bulletin,2006,51(9):1069-1077. |
| |
| Authors: | Ying Lu Chenxi Gao Bin Han |
| |
| Institution: | National Center for Gene Research, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200233, China;Graduate School of the Chinese Academy of Sciences, Beijing 100039, China;National Center for Gene Research, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200233, China;Graduate School of the Chinese Academy of Sciences, Beijing 100039, China;Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, China;National Center for Gene Research, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200233, China;Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, China |
| |
| Abstract: | The formation of eukaryotic mRNAs involves the cleavage and polyadenylation of pre-mRNAs. To investigate the sequence requirement
of putative polyadenylation signals (PASs), poly(A) sites and downstream elements (DUEs) in 3′-end-processing in rice, we
compared expressed sequences tags (ESTs) with poly(A) extremity to full-length cDNA sequences and constructed a database of
12969 pre-mRNA sequences in −40–+40 nt surrounding the poly(A) sites, which were from 9953 genes. The alternative poly(A)
sites were revealed in approximately 25% of mRNAs. Nearly 80% of pre-mRNAs showed stringent requirement of the YA (CA or UA)
at poly (A) sites for polyadenylation. About 7.9% had the AAUAAA signals on −40–−1 nt upstream of the poly(A) sites. Over
60% of mRNAs probably used the one-or two-base variants of AAUAAA hexamers as their PASs in 3′ fragments. The single-base
variants of AAUGAA revealed the high frequency in 11.5% of 3′ fragments. The DUEs were detected in 90% of pre-mRNAs, especially
more than half of the pre-mRNAs with multi-base variants of AAUAAA had the DUEs surrounding the poly(A) site. The location
of DUE is also important for defining the cleavage site. Although most of the rice pre-mRNAs did not contain AAUAAA signal,
the existence of downstream elements ensured the efficiency of cleavage-polyadenylation |
| |
| Keywords: | polyadenylation signals U-rich downstream elements cDNA expressed sequences tags RNA processing rice |
| 本文献已被 CNKI 维普 万方数据 SpringerLink 等数据库收录！ |
| 点击此处可从《中国科学通报(英文版)》浏览原始摘要信息 |
| 点击此处可从《中国科学通报(英文版)》下载免费的PDF全文 |
|
