| 高活性牛肠碱性磷酸酶的制备 |
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| 引用本文： | 蒋益众,邱耀进,杨曜中,郭春祥.高活性牛肠碱性磷酸酶的制备[J].应用科学学报,1984,2(2):178-183. |
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| 作者姓名： | 蒋益众 邱耀进 杨曜中 郭春祥 |
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| 作者单位： | 1. 上海科技大学;2. 华东化工学院;3. 上海医学化验所 |
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| 摘 要： | 本文叙述一种从小牛肠粘膜制备碱性磷酸酶的方法.主要包括用正丁醇提取,丙酮和硫酸铵分级,DEAE纤维素离子交换层析和Sephadex凝胶过滤.所得制剂比活为845单位/毫克蛋白;活力回收29%;聚丙烯酰胺凝胶电泳显示单一性活力区带.
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| 收稿时间： | 1982-04-27 |
THE PREPARATION OF HIGH ACTIVITY ALKALIN PHOSPHATASE FROM CALF INTESTINAL MUCOSE |
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| JIANG YIZHONG,QIOU YAOJIU,YANG YANZHONG,QUO CHUNXIANG.THE PREPARATION OF HIGH ACTIVITY ALKALIN PHOSPHATASE FROM CALF INTESTINAL MUCOSE[J].Journal of Applied Sciences,1984,2(2):178-183. |
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| Authors: | JIANG YIZHONG QIOU YAOJIU YANG YANZHONG QUO CHUNXIANG |
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| Institution: | 1. Shanghai University of Science and Technology;2. East China Institute of Chemical Technology;3. Shanghai Medical Laboratory |
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| Abstract: | This paper presents a method for the preparation of alkaline phosphatase (EC 3.1.3.1) from calf intestinal mueose. The method involves extraction of the mucose with butanol, fractionation with acetone and then (NH4)2SO4, DEAE-cellulose ion-exchange column chromatography and Sephadex-gel filtration. The specific activity of the enzyme preparation was about 845 units mg of protein. The recovery of activity was 29%. Only one activity band was shown by the polyacrylamide gel electrophoresis. |
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