| 植物多酚氧化酶活性测定方法的改进 |
| |
| 引用本文： | 李忠光,龚明.植物多酚氧化酶活性测定方法的改进[J].云南师范大学学报(自然科学版),2005,25(1):44-45,49. |
| |
| 作者姓名： | 李忠光 龚明 |
| |
| 作者单位： | 云南师范大学生命科学学院,云南,昆明,650092 |
| |
| 基金项目： | 国家自然科学基金(3986000)，云南省自然科学基金(98C002Z) |
| |
| 摘 要： | 文章介绍了多酚氧化酶反应产物邻醌的吸收光谱和酶测定反应的动力学分析,确定了邻醌的最高吸收光谱和酶测定的适宜参数。这一测定方法与原方法1]相比,提高了多酚氧化酶测定的准确性、重复性和可比性,使灵敏度提高了5倍。
|
| 关 键 词： | 改进 多酚氧化酶 动力学曲线 |
| 文章编号： | 1007-9793(2005)01-0044-02 |
Improvement of measurement method of polyphenol oxidase activities in plant |
| |
| LI Zhong-guang,Gong Ming.Improvement of measurement method of polyphenol oxidase activities in plant[J].Journal of Yunnan Normal University (Natural Sciences Edition),2005,25(1):44-45,49. |
| |
| Authors: | LI Zhong-guang Gong Ming |
| |
| Abstract: | This paper introduced absorption spectrum of o-quinone and kinetics curve of polyphenol oxidase reaction, the wavelength of maximum absorbance spectrum and the suitable parameters were selected for the measurement of polyphenol oxidase reaction. This method enhanced the accuracy, reproducibility and comparability for the polyphenol oxidase measurement and sensitivity enhanced five-fold with original method. |
| |
| Keywords: | improvement polyphenol oxidase kinetics curve |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
