| 山苦菜无性系建立的研究 |
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| 引用本文： | 张卓,王艳,李慧,王晓旭,姜长阳.山苦菜无性系建立的研究[J].河南大学学报(自然科学版),2012,42(2):187-191. |
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| 作者姓名： | 张卓 王艳 李慧 王晓旭 姜长阳 |
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| 作者单位： | 辽宁师范大学生命科学学院,辽宁大连,116081 |
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| 基金项目： | 辽宁省高等教育教学改革资助项目(20090304);辽宁师范大学教学改革资助项目(LSJG:20090108) |
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| 摘 要： | 以山苦菜无芽嫩茎为外植体诱导愈伤组织,经过继代培养后,以生长状态良好的愈伤组织进行分化培养,继续进行分化苗的生根培养,建立起山苦菜的无性系.结果表明:MS+BA(1.0mg/L)+NAA(0.1mg/L)+蔗糖(30g/L)是愈伤组织诱导和继代培养的最佳培养基;愈伤组织分化的最佳培养基为1/2MS+蔗糖(15g/L);诱导不定芽生根的最佳培养基是1/4MS+IAA(0.2mg/L)+蔗糖(20g/L);山苦菜试管苗移栽的最佳基质为1/2园土+1/2河沙.
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| 关 键 词： | 山苦菜 组织培养 无性系 |
Study on the Clone Construction of Ixeris chinensis |
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| ZHANG Zhuo
,
WANG Yan
,
LI Hui
,
WANG Xiao-xu
,
JIANG Chang-yang.Study on the Clone Construction of Ixeris chinensis[J].Journal of Henan University(Natural Science),2012,42(2):187-191. |
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| Authors: | ZHANG Zhuo WANG Yan LI Hui WANG Xiao-xu JIANG Chang-yang |
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| Institution: | (College of Life Science,Liaoning Normal University,Liaoning Dalian 116081,China) |
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| Abstract: | Tender stems without buds of Ixeris chinensis were used as explants to induce callus,the callus of best growth were cultivated for differentiation.With further rooting cluture of differentiated seedlings,the clone of Ixeris chinensis was established.The results indicated that MS+BA(1.0 mg/L)+ NAA(0.1 mg/L)+ sucrose(30 g/L)was the perfect medium for callus inducing and callus multiplication;the best medium for callus differentiation was 1/2 MS+ sucrose(15 g/L);the ideal medium for rooting of adventitious buds was 1/4 MS+IAA(0.2 mg/L)+ sucrose(20 g/L);the best substrate for transplanting was 1/2 clays from garden +1/2 sands. |
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| Keywords: | Ixeris chinensis culture of tissue clone |
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