| 盾叶薯蓣毛状根的诱导与薯蓣皂甙元的生产 |
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| 引用本文： | 陈永勤,沈君豪,潘军.盾叶薯蓣毛状根的诱导与薯蓣皂甙元的生产[J].湖北大学学报(自然科学版),2009,31(1):74-78. |
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| 作者姓名： | 陈永勤 沈君豪 潘军 |
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| 作者单位： | 湖北大学,生命科学学院,湖北,武汉,430062
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| 基金项目： | 湖北省教育厅资助项目，国家自然科学基金 |
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| 摘 要： | 报道盾叶薯蓣形成毛状根诱导的方法及不同毛状根系间生长速率与薯蓣皂甙元含量的差异．结果表明，盾叶薯蓣的幼叶和茎段难被R1601、A4和LBA9402等发根农杆菌菌株诱导出毛状根，但愈伤组织则比较容易；34%的被R1601菌株感染、并在添加200μmol／L乙酰丁香酮的共培养基上培养3d的愈伤可以在30d内形成毛状根；不同的毛状根系在生长速率与薯蓣皂甙元含量方面存在显著差异．
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| 关 键 词： | 盾叶薯蓣 薯蓣皂甙元 发根农杆菌 毛状根 |
Hairy root induction of Dioscorea zingiberensis and diosgenin production |
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| CHEN Yong-qin,SHEN Jun-hao,PAN Jun.Hairy root induction of Dioscorea zingiberensis and diosgenin production[J].Journal of Hubei University(Natural Science Edition),2009,31(1):74-78. |
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| Authors: | CHEN Yong-qin SHEN Jun-hao PAN Jun |
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| Institution: | School of Life Science;Hubei University;Wuhan 430062;China |
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| Abstract: | Dioscorea zingiberensis is the most important medicinal plant used for production of diosgenin in China.This paper reports that the effects of explant types,strains of Agrobacterium rhizogenes and acetosyringone concentrations on the induction of hairy roots of D.zingiberensis,and the growth rates and diosgenin contents of different hairy root clones.The results showed that hairy roots could not be induced by A.rhizogenes strain R1601,A4 or LBA9402 from both the young leaves and stems of D.zingiberensis,but... |
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| Keywords: | Dioscorea zingiberensis diosgenin Agrobacterium rhizogenes hairy root |
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