| 1株海洋石油降解菌的筛选鉴定及其固定化研究 |
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| 引用本文： | 关晓燕,董颖,王摆,王召会,陈仲,杨爱馥,姜北,高杉,蒋经伟,周遵春,郭大立,张戈.1株海洋石油降解菌的筛选鉴定及其固定化研究[J].辽宁师范大学学报(自然科学版),2013(3):400-405. |
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| 作者姓名： | 关晓燕 董颖 王摆 王召会 陈仲 杨爱馥 姜北 高杉 蒋经伟 周遵春 郭大立 张戈 |
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| 作者单位： | [1]辽宁省海洋水产科学研究院辽宁省海洋环境监测总站辽宁省海洋水产分子生物学重点实验室,辽宁大连116023 [2]辽宁师范大学城市与环境学院,辽宁大连116029 |
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| 基金项目： | 辽宁省海洋渔业科技项目（2011010）;辽宁省科技计划项目（2011203005） |
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| 摘 要： | 从受石油污染的海洋沉积物中分离得到1株能以柴油为唯一碳源生长的菌株LHOD-2,通过形态特征、生理生化及16SrDNA序列分析,初步鉴定菌株LHOD-2属于埃氏假交替单胞菌(Pseudoalteromonas espejiana).利用聚氨酯泡沫为载体制备固定化菌,并对其降解特性进行考察.实验结果表明,菌株LHOD-2的最佳生长降解条件为温度25~30℃,pH 7.0~8.0,盐度(体积分数)3%~3.5%,转速150r/min.在最佳条件下,LHOD-2对800mg/L柴油在120h内的降解率达到85%(质量分数).聚氨酯泡沫载体具有大孔网状结构,利于菌株生长和传质,固定化菌降解柴油的速率明显高于游离菌.
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| 关 键 词： | 柴油 埃氏假交替单胞菌 固定化 生物降解 |
Study on isolation, identification and immobilition of a marine oil-degrading strain |
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| GUAN Xiaoyan,DONG Ying,WANG Bai,Vf,NG Zhaohui,CHEN Zhong,YANG Ai fu,JIANG Bei,GAO Shan,JIANG Jingwei,ZHOU Zunchun,GUO Dali,ZHANG Ge.Study on isolation, identification and immobilition of a marine oil-degrading strain[J].Journal of Liaoning Normal University(Natural Science Edition),2013(3):400-405. |
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| Authors: | GUAN Xiaoyan DONG Ying WANG Bai Vf NG Zhaohui CHEN Zhong YANG Ai fu JIANG Bei GAO Shan JIANG Jingwei ZHOU Zunchun GUO Dali ZHANG Ge |
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| Institution: | 2 (1. Liaoning Ocean and Fisheries Science Research Institute Liaoning Marine Environmental Monitoring General Station Liaoning Key Lab of Marine Fisheries Molecular Biology, Dalian 116023,China 2. College of Urban and Environment, Liaoning Normal University,Dalian 116029,China) |
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| Abstract: | Strain LHOD-2 was isolated from the petroleum-polluted marine sediments, which can utilize diesel oil as sole carbon source for growth. It was identified as Pseudoalteromonas espejiana, according to its morphology and biochemical characteristics, and 16S rDNA sequence analysis. Then strain LHOD-2 was immobilized on polyurethane foam for investigating the characteristics of immobi- lized cells. The results showed that the optimal conditions for diesel oil degradation by strain LHOD- 2 were 25-30 ℃, pH of 7.0-8.0, 3%-3.5% salinity and rotate velocity of 150 r/min. And there was 85% removal rate within 120 h for 800 mg/L diesel oil under the optimal conditions. It was sug- gested that the macro-reticular structure of polyurethane was beneficial for diesel oil degradation by accelerating the strain growth and mass transfer. |
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| Keywords: | diesel oil Pseudoalteromonas espejiana immobilization biodegradation |
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