黄秋葵愈伤组织培养及再生体系建立的研究

以具有杂种黄蜀葵嫩茎为材料,采用组织培养的方法,进行了嫩茎的愈伤组织诱导、分化,不定芽的生根以及试管苗的生根继代增殖培养、移栽与定植的研究.结果表明:嫩茎愈伤组织诱导培养和继代增殖培养的理想培养基是MS+6-BA0.75mg/L+2,4-D2.1 mg/L;愈伤组织分化培养的理想培养基是MS+AgNO31.0 mg/L+NAA0.1mg/L+ZT1.6mg/L;1/3MS+蔗糖10g/L+IAA0.4mg/L是不定芽生根培养的理想培养基;试管苗生根继代增殖培养的理想培养基是1/3MS+ABT0.6mg/L+蔗糖10g/L+IAA0.4mg/L.试管苗移栽成活率为93.9%,定植成活率为97.5%.定植的试管苗保持了杂种黄秋葵所有植物学性状和杂种优势.

Callus culture and establishment of regeneration system of Abelmoschus esculentus

In order to preserve the heterosis of germchits and meet the need of germchits for cultiva- tion,the tender stems of Abelmoschus esculentus were used as materials to do the research by using tissue culture methods. The study was mainly on callus culture and differentiation of tender stems, rooting of adventitious bud,rooting and subculturing of tube seedlings,and tube seedlings transplan- ting colonization. The results demonstrated that MS＋6-BA0.75 mg/L＋2,4-D2.1 mg/L was the op- timum medium for the callus culture and subculturing of the tender stems. MS ＋AgNO3 1.0 rag/L＋ NAA0.1 rag/L＋ ZT1.6 mg/L was the optimum medium for the differentiation cultivation of callus tissue. 1/3MS ＋sucrose 10 g/L＋IAA 0.4 mg/L was the suitable medium for rooting culture of ad- ventitious bud. 1/3MS＋ABT 0.6 mg/L＋sucrose 10 g/L＋IAA 0.4 mg/L was the optimum medium for rooting and subculture of tube seedlings. The transplanting survival rate of tube seedlings was 93.9% and stable planting survival rate was 97.5% Colonization of plantlets maintained all the bo- tanical character and the heterosis of Abelmoschus esculentus