庆大霉素/水杨酸/壳聚糖复方纳米粒的制备及体外释放

通过壳聚糖与三聚磷酸钠的离子交联作用制备了具有拮抗庆大霉素耳毒性功效的庆大霉素/水杨酸复方壳聚糖纳米粒。用紫外分光光度计、纳米粒度仪和Zeta电位仪、透射电子显微镜、扫描电子显微镜、傅里叶变换红外光谱仪和X射线衍射仪等考察了壳聚糖纳米粒的粒径、zeta电位、形态、载药能力及体外释放行为。结果显示复方纳米粒为球形,平均粒径为40 nm;庆大霉素与水杨酸的包封率分别为(91.24±0.24)%和(80.75±0.15)%,载药量分别为(34.15±1.02)%和(38.35±0.48)%;在体外释放试验中,庆

Preparation of the gentamicin and salicylic acid-loaded chitosan nanoparticles and its in vitro releasing investigation

Gentamycin (GM) and salicylic acid (SA) loaded chitosan (CS) nanoparticles are prepared and used to inhibit the ototoxicity of GM. Nanoparticles are prepared via the cross-linking method by CS and sodium tripolyphosphate. The characteristics of nanoparticles such as size, zeta potential, shape, loading capacity, and in vitro release profiles are determined. The nanoparticles are spherical in shape with an average diameter of 40 nm. The entrapment efficient of GM and SA is (91.24±0.24)% and (80.75±0.15)% respectively, and loading capacity are (34.15±1.02)% and (38.35±0.48)%. The drug release shows good sustained-release effect and follows ambiexponent kinetic equation. It demonstrates that GM and SA loaded CS nanoparticles have promising potential effect on antagonism ototoxicity of GM, and the nephrotoxicity may be also decreased through the expected sustained-release characteristics of CS nanoparticles.