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| 烛缸法分离培养及冷冻法保存幽门螺杆菌 | |
| 引用本文： | 万学勤,周鹏志,马峰,李万可兰,谭淑仪,邝佳. 烛缸法分离培养及冷冻法保存幽门螺杆菌[J]. 佛山科学技术学院学报(自然科学版), 2011, 29(5): 82-85 |
| 作者姓名： | 万学勤 周鹏志 马峰 李万可兰 谭淑仪 邝佳 |
| 作者单位： | 1. 佛山科学技术学院检药系,广东佛山,528000 2. 广州医学院第三附属医院,广东广州,510150 3. 南方医科大学南方医院,广东广州,510515 |
| 基金项目： | 广东省自然科学基金项目(9152800001000022); 广东省科技厅社会发展项目(2010B031600150) |
| 摘 要： | 目的研究幽门螺杆菌（H elicobacter py lori,H py lori）简易分离培养及保存方法,构建H py lori小型菌库。方法首先采用H py lori选择培养基,以烛缸法分离培养不同来源的标本,然后以革兰染色显微镜形态检查及生化试验鉴定为H py lori,最后把鉴定为H py lori的纯培养物,... |
| 关 键 词： | 幽门螺杆菌 分离培养 菌种 菌种收藏 |
Candle jar fractional cultivation and cryopreservation of Helicobacter pylori |
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| WAN Xue-qin,ZHOU Peng-zhi,MA Feng,LI Wan-kelan,TAN Shu-yi,KUANG Jia. Candle jar fractional cultivation and cryopreservation of Helicobacter pylori[J]. Journal of Foshan University(Natural Science Edition), 2011, 29(5): 82-85 | |
| Authors: | WAN Xue-qin ZHOU Peng-zhi MA Feng LI Wan-kelan TAN Shu-yi KUANG Jia |
| Affiliation: | WAN Xue-qin1,ZHOU Peng-zhi2,MA Feng3,LI Wan-kelan1,TAN Shu-yi1,KUANG Jia1(1.Department of Laboratory phartmacy,Foshan University,Foshan 528000,China,2.The third Affiliated Hospital of Guangzhou Medical University,Guangzhou 510150,3.Nanfang Hospital,Southern Medical University,Guangzhou 510515,China) |
| Abstract: | Objects To study the simple method for the separation and reservation of Helicobacter pylori(H pylori) and construct a small storage of H pylori.Method Firstly,the candle jar method was used to separate samples of H pylori from different sources by H pylori selective medium,Secondarily,the Gram microscopy test and biochemical experiment were used to identify H pylori.Thirdly,the pure H pylori was reserved in three different preservation solutions(brucella broth,50% glycerol serum,50% glycerol egg white) whi... |
| Keywords: | Helicobacter pylori fractional cultivation bacterial isolates type culture collection |
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