| 基于鲁米诺的流动注射化学发光法测定硫酸链霉素 |
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| 引用本文： | 蔡富春,黄玉明,章竹君.基于鲁米诺的流动注射化学发光法测定硫酸链霉素[J].西南师范大学学报(自然科学版),2004,29(1):79-83. |
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| 作者姓名： | 蔡富春 黄玉明 章竹君 |
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| 作者单位： | 西南师范大学,化学化工学院,重庆,400715;西南师范大学,分析科学研究所,重庆,400715 |
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| 摘 要： | 建立了一种简易、快速测定硫酸链霉素的流动注射化学发光法.本法基于在强碱性介质中,硫酸链霉素能增强铁氰化钾氧化鲁米诺产生化学发光,加入亚铁氰化钾能大大降低这一体系的背景发光强度.在优化的实验条件下该法测定硫酸链霉素的线性范围为5～1000μg/mL,检出限(3σ)为0 6μg/mL.对100μg/mL的硫酸链霉素进行11次平行测定,其RSD为2 2%.将本法用于硫酸链霉素制剂及合成样品的分析,结果令人满意.
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| 关 键 词： | 化学发光 硫酸链霉素 药物分析 |
Chemiluminescent Determination on a Luminol Reaction of Streptomycin Sulfate by Flow Injection Analysis |
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| Abstract.Chemiluminescent Determination on a Luminol Reaction of Streptomycin Sulfate by Flow Injection Analysis[J].Journal of Southwest China Normal University(Natural Science),2004,29(1):79-83. |
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| Authors: | Abstract |
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| Abstract: | A chemiluminescence(CL) flow system is described for the determination of streptomycin based on the chemiluminescence emission produced upon mixing a hexacyanoferrate(Ⅲ) solution with an alkaline luminol solution. The emission intensity is enhanced by streptomycin in the presence of potassium hexacyanoferrate(Ⅱ) as suppressor reagent. The system responds linearly to streptomycin concentration in the range 5-1 000 μg/mL with a detection limit(3σ) of 0.6 μg/mL. Relative standard deviation (RSD) is 2.2% for 100 μg/mL streptomycin(n=11). The system has been successfully applied to the determination of streptomycin in pharmaceutical preparations and synthetical samples. |
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| Keywords: | chemiluminescence streptomycin pharmaceutical analysis |
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