丙烯腈水合酶反应动力学与酶失活动力学研究

在以丙烯腈为原料，微生物转化生产丙烯酰胺的过程中，酶催化反应是过程的关键。为了了解酶催化的动力学，以自由细胞的酶为催化剂，进行了腈水合酶的反应动力学和失活动力学的研究。首先研究了菌体浓度、温度、pH值、丙烯腈浓度、丙烯酰胺浓度等对腈水合酶催化反应速度的影响。结果表明，在这些因素中，温度和丙烯酰胺浓度是最主要的影响因素。经过对不同温度下的反应速度的研究，得到腈水合酶水合反应的活化能为昏60．87kJ／mol，酶失活反应的活化能为89．36kJ／mol。

Enzyme Catalytic Kinetics and De-active Dynamics of Nitrile Hydratase

The hydration reaction by microbial method is the crisis of the procedure of acrylamide production from acrylonltrile. The research studied the enzyme talytic kinetics and deactive kinetics of nitrle hydratase in the type of free cell. The effects of the concentration of cells, the temperature, pit value, the concentration of acrylonitrile and the concentration of acrylamide on the activity of niltrile hydratase were investigated. The result was that the temperature and the concentration of acrylamide are the most important amoltg these factors. It was found that the activation energy of the hydration of NHase was Ea=60.87 kJ/mol, the deactivation energy was 89.36 kJ/mol.