Density gradient centrifugation of cells separated from multicellular tumor spheroids |
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| Authors: | C. P. Sigdestad D. J. Grdina K. Ando |
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| Affiliation: | (1) Section of Experimental Radiotherapy, University of Texas System Cancer Center, M. D. Anderson Hospital & Tumor Institute, 6723 Bertner Avenue, 77030, Texas, Houston, USA;(2) Present address: Department of Therapeutic Radiology, University of Louisville, 500 S. Floyd St., 40202 Louisville, Kentucky, USA |
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| Abstract: | Summary Cells from a murine fibrosarcoma (FSa) have been grown in vitro as multicell tumor spheroids (MTS). The growth rate of these MTS was determined. Follwing selected periods of growth, MTS were made into a single cell suspension and separated on linear density gradients of Renografin. While only 1 population of cells was separated from small spheroids (400  m diameter), at least 3 subpopulations of tumor cells were separated and isolated from large spheroids (800 m in diameter).This investigation was supported in part by grants numbered CA-18628 and CA-06294, awarded by the National Cancer Institute, DHEW. |
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