Effects of antisense CENP-B on the proliferation of HeLa (Tet-off) cells |
| |
Authors: | Qianjin Liang Qian Wang Haiyan Lin Song Luo Chunmei Wang Dacheng He Yongchao Wang |
| |
Institution: | (1) College of Life Sciences, Beijing Normal University, 100875 Beijing, China |
| |
Abstract: | In order to study the change of the expression of centromere protein B (CENP-B) caused by antisense transfection, proto-eukaryotically
expressed fused protein GST-CENP-B (65 ku) was injected into mouse, and a peculiar anti-CENP-B serum MaCenpB was collected.
A strain of transfected HeLa Tet-off cell HaCb, which contains antisense CENP-B expressing vector pBI-EGFP-as-CenpB, was prepared. Northern blot and Western blot were used to analyze the repression of
internal CENP-B in transfected cells. According to the growth curve, the proliferation of HeLa (Tet-off) is repressed by antisense CENP-B, and the multiplication time is prolonged for 32.81 h. The analysis of flow cytometry revealed that, compared with HeLa (Tet-off),
the G1 cell population of HaCb is increased (ΔG1= 9%) while S fraction is decreased (ΔS = 11%), but the G2/M phase is nearly unchanged (ΔG2/M=3%). In the meanwhile, the mitotic index of HaCb declines greatly compared with that of HeLa (Tet-off). Immunofluorescence
showed that the assembling of centromeres in HaCb cell is arrested. These results suggest that a normal expression of CENP-B may be necessary for cell proliferation. |
| |
Keywords: | centromere proteins CENP-B HeLa (Tet-off) cell |
本文献已被 SpringerLink 等数据库收录! |
| 点击此处可从《中国科学通报(英文版)》浏览原始摘要信息 |
| 点击此处可从《中国科学通报(英文版)》下载免费的PDF全文 |
|