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| 固定化人工膜与非膜蛋白亲和作用研究 | |
| 引用本文： | 江晶,王珊珊,邓玉林.固定化人工膜与非膜蛋白亲和作用研究[J].东北大学学报(自然科学版),2010,31(5):757-760. |
| 作者姓名： | 江晶 王珊珊 邓玉林 |
| 作者单位： | 1. 东北大学,机械工程与自动化学院,辽宁,沈阳,110004 2. 山东省科学院分析测试中心,山东,济南,250014 3. 北京理工大学,生命科学与技术学院,北京,100081 |
| 摘 要： | 由于膜蛋白在细胞中含量较低、提取纯化工作复杂且价格昂贵,因此筛选与类生物膜结构具有强亲和作用的非膜蛋白研究具有重要意义.以固定化人工膜(IAM)作为生物膜模型,将其填充至毛细管中作为微柱液相色谱的固定相.选取10种常见易得的非膜蛋白作为研究对象,分别在酸性、中性、碱性条件下,研究这些蛋白与固定化人工膜的相互作用.结果表明中性的磷酸盐缓冲溶液是蛋白与IAM结合的最佳环境,在此条件下有5种蛋白表现出与IAM具有较强的亲和性. |
| 关 键 词： | 固定化人工膜 非膜蛋白 微柱液相色谱 等电点 生物膜 亲和作用 |
Study on the Affinity Between IAM and Non-membrane Proteins |
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| JIANG Jing,WANG Shan-shan,DENG Yu-lin.Study on the Affinity Between IAM and Non-membrane Proteins[J].Journal of Northeastern University(Natural Science),2010,31(5):757-760. | |
| Authors: | JIANG Jing WANG Shan-shan DENG Yu-lin |
| Institution: | (1) School of Mechanical Engineering and Automation, Northeastern University, Shenyang 110004, China; (2) Analysis and Test Center, Shandong Academy of Sciences, Jinan 250014, China; (3) School of Life Science and Technology, Beijing Institute of Technology, Beijing 100081, China |
| Abstract: | Owing to the low content of membrane protein in cell and its complicated extraction/purification process with high cost, the membrane-related study on screening the non-membrane proteins which have strong affinity for biomembrane is of significance. With the IAM taken as a biomembrane model, it was filled into a capillary as the stationary phase in micro-column liquid chromatography. Then, ten kinds of common proteins were selected for investigating the interactions between IAM and then under acidic, neutral and alkali conditions. The results showed that the neutral phosphate buffer solution provides the environment where the proteins will combine best with IAM, thus enabling five kinds of proteins to provide strong affinity for IAM. |
| Keywords: | IAM(immobilized artificial membrane) non-membrane protein micro-column liquid chromotography isoelectric point biomembrane affinity |
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