Affinity capillary electrophoresis in biomolecular recognition |
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| Authors: | Y-H Chu C C Cheng |
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| Institution: | (1) Department of Chemistry, The Ohio State University, 100 West 18th Avenue, Columbus (Ohio 43210, USA), Fax +1 614 292 1685, e-mail: chu@chemistry.ohio-state.edu, US |
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| Abstract: | Affinity capillary electrophoresis is a new method for studies of biomolecular recognition. Applications reported in the
literature include chiral separation of racemic biomolecules, measurement of binding constants, estimation of kinetic on-
and off-rate constants, determination of binding stoichiometries (a useful tool in examining electrostatic interactions),
estimation of effective charges and molecular weights of proteins, characterization of enzymatic activities and library screening
for tight-binding drug candidates in solution. This technique demands only small amounts of sample (nanolitre injection volumes,
picograms of proteins), involves no radiolabelled materials or chemically immobilized ligands, and does not require changes
in spectroscopic characteristics upon binding. This paper reviews the most recent applications of affinity capillary electrophoresis
and its use in the analysis of biomolecules.
Received 9 January 1998; received after revision 27 February 1998; accepted 3 March 1998 |
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| Keywords: | , Affinity capillary electrophoresis, receptor-ligand binding interaction, molecular recognition, combinatorial library,,,,,screening, protein, |
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