| 多种金属离子对白蜡虫碱性磷酸酶活性的影响 |
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| 引用本文： | 赵欣平,舒畅,杨芳,刘克武,喻东.多种金属离子对白蜡虫碱性磷酸酶活性的影响[J].四川大学学报(自然科学版),2002,39(1):132-135. |
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| 作者姓名： | 赵欣平 舒畅 杨芳 刘克武 喻东 |
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| 作者单位： | 四川大学生命科学学院,成都610064 |
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| 摘 要： | 研究了多种金属离子对白蜡虫碱性磷酸酶活性的影响。结果表明：Li^ ,Na^ ,K^ 对酶活力没有影响，Ca^2 ，Mg^2 ,Ba^2 ,Mn^2 ,Co^2 有激活作用；而Zn^2 ,Cu^2 ,Cd^2 ,Pb^2 有抑制作用，Ca^2 是有效的激活剂，表现为非竞争性激活效应，Cu^2 为有效的抑制剂，表现为非竞争性抑制效应，酶液经EDTA透析处理后活力完全丧失，失活的酶可用加入Zn^2 和Ca^2 复活。
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| 关 键 词： | 白蜡虫 碱性磷酸酶 金属离子 酶活性 非竞争性激活效应 非竞争性抑制效应 铜 钙 |
| 文章编号： | 0490-6756(2002)01-0132-04 |
Influence of Metal Ions on the Activity of AlkalinePhosphatase from Ericerus Pela (Chavannes) |
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| ZHAO Xin ping,SHU Chang,YANG Fang,LIU Ke wu,YU Dong.Influence of Metal Ions on the Activity of AlkalinePhosphatase from Ericerus Pela (Chavannes)[J].Journal of Sichuan University (Natural Science Edition),2002,39(1):132-135. |
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| Authors: | ZHAO Xin ping SHU Chang YANG Fang LIU Ke wu YU Dong |
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| Abstract: | The survey of influence of metal ions on the activity of alkaline phosphatase (AKP) from Ericerus pela (Chavannes) showed that Li +, Na + and K + had not any effect on the enzyme activity. Ca 2+ , Mg 2+ , Ba 2+ , Mn 2+ , Co 2+ activated the enzyme. The calcium ion was a more efficacious activator and the effect was classified as noncompetitive type. Zn 2+ , Cu 2+ , Cd 2+ and Pb 2+ inhibited the enzyme. The copper ion was a more efficacious inhibitor. The inhibition of copper ion on the enzyme was found to be of noncompetitive type. After dialysistreatment with EDTA, the enzyme was totally inactivated. The inactivated enzyme could be reactivated by adding Zn 2+ and Ca 2+ . |
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| Keywords: | Ericerus pela (Chavannes) alkaline phosphatase metal ion |
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