| 5种蟋蟀的RAPD研究初报(直翅目:蟋蟀总科) |
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| 引用本文： | 田英芳,郑哲民.5种蟋蟀的RAPD研究初报(直翅目:蟋蟀总科)[J].陕西师范大学学报,2002,30(2):91-93. |
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| 作者姓名： | 田英芳 郑哲民 |
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| 作者单位： | [1]西安交通大学医学院，陕西西安710061 [2]陕西师范大学动物研究所，陕西西安710062 |
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| 基金项目： | 国家自然科学基金资助项目 (395 70 10 ) |
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| 摘 要： | 应用RAPD技术对5种蟋蟀的基因组DNA进行扩增，从10种随机引物中筛选出一种引S8可以对5个种扩增出稳定且相异的DNA图谱，每个种的DNA扩增条带的相对分子质量为：长颚斗蟋V.aspersus S8-1900,1510,1320,830,740,370；小斗蟋V.parvus S8-1900,1150,830,600,350；石首棺头蟋L.equestris S8-1900,960,560，多伊棺头蟋，L.doenitzi S8-1900,800；黄脸油葫芦T.emma S8-1490,1290,1180。结果表明，引物S8可用于5种蟋蟀的分类鉴定。
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| 关 键 词： | RAPD 直翅目 蟋蟀总科 昆虫分类 DNA图谱 相对分子质量 长颚斗蟋 小斗蟋 |
| 文章编号： | 1001-3857(2002)02-0091-03 |
| 修稿时间： | 2001年6月9日 |
Random amplified polymorphic DNA in five species of crickets (Orthoptera:Grylloidea) |
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| TIAN Ying fang ,ZHENG Zhe min.Random amplified polymorphic DNA in five species of crickets (Orthoptera:Grylloidea)[J].Journal of Shaanxi Normal University: Nat Sci Ed,2002,30(2):91-93. |
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| Authors: | TIAN Ying fang ZHENG Zhe min |
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| Institution: | TIAN Ying fang 1,ZHENG Zhe min 2 |
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| Abstract: | The RAPD protocols is first used to study the genomic DNA of 5 species of crickets. The genomic DNA of 5 species of crickets is amplified with 10 different oligo nucleotide 10 mer primer. Primer S8 is screened by RAPD PCR and five separate DNA maps are obtained through amplifying the genomic DNA of the crickets, lengths of the DNA bands of each map: Velarifictorus. asperses S8-1 900, 1 510 , 1 320, 830, 740, 370, V.parvus S8-1 900, 1 150 , 830, 600, 350, L.equestris S8-1 900, 960, 560, L.doenitzi S8-1 900, 800, T.emma S8-1 490, 1 290, 1 180. The study also shows that amplified DNA exist sexual and individuals differences, but these differences distinctly differ from those among the species. The test results are stable and reproducible. Prime S8 could be used for the identification of five species of crickets. |
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| Keywords: | RAPD cricket insect taxonomy |
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