| 中华猕猴桃蛋白酶羧基的化学修饰 |
| |
| 引用本文： | 林沁瑛,陈海.中华猕猴桃蛋白酶羧基的化学修饰[J].厦门大学学报(自然科学版),1992,31(4):452-454. |
| |
| 作者姓名： | 林沁瑛 陈海 |
| |
| 作者单位： | 厦门大学生物学系
(林沁瑛，陈海，刘纳真)，厦门大学生物学系(颜思旭) |
| |
| 摘 要： | 有关中华猕猴桃蛋白酶(Actinidin,E C 3、4.22.14)催化功能基团的研究表明:CYSH—25、His—161为酶活性部位必需基团,而Lys和Trp残基为酶活力表现所必需。本文中报道应用水溶性碳二亚胺(EDC)修饰动力学方法,进一步探讨羧基
|
| 关 键 词： | 蛋白酶 羧基 修饰 催化 |
Modification of Essential Carboxyl Group in Actinicin from Actinidia Chinensis |
| |
| Lin Qinying Chen Hai Liu Nazhen Yan Sixu.Modification of Essential Carboxyl Group in Actinicin from Actinidia Chinensis[J].Journal of Xiamen University(Natural Science),1992,31(4):452-454. |
| |
| Authors: | Lin Qinying Chen Hai Liu Nazhen Yan Sixu |
| |
| Institution: | Dept. of Biol. |
| |
| Abstract: | Actinidin was isolated and purified from fruits of wild Actinidia chinensis. The enzyme can be repidly inactivated by water-soluble carbodiimide (1-ethy-3-(3-dimethyl aminoprgpyl) carbodi-imide, EDC). The chemical modification kinetic data indicated that only one carboxyl group was related to the enzyme activity. |
| |
| Keywords: | Modification Carboxyl group Actinidin |
| 本文献已被 CNKI 维普 等数据库收录！ |
